Angiotensin I Converting Enzyme 1 Antikörper

Produktnummer ABIN111943

Dieser Maus Monoklonal Antikörper detektiert spezifisch Angiotensin I Converting Enzyme 1 in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Angiotensin I Converting Enzyme 1 Antikörper (ABIN111943)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Angiotensin I Converting Enzyme 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CG2

Produktdetails anti-Angiotensin I Converting Enzyme 1 Antikörper

Spezifität: Angiotensin converting Enzyme (ACE), denatured. The epitope recognized is localized on the NH2-terminal side of the molecule. Topographically the degree of expression correlates with the proportion of smooth muscle cells and elastic fibres of the vessel wall. ACE is predominantely expressed by small muscular arteries and by arterioles, whereas small and large veins do not usually express ACE. However, no ACE could be detected in the endothelial cells of kidney. Constitutively ACE is expressed on endothelial cells in different diseases like hypertension, myocardial infarction, sarcoidosis, diabetes.

Aufreinigung: Protein A chromatography.

Immunogen: Purified ACE from human lung and kidney.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry on Paraffin sections: 8 μg/mL (1: 50), microwavepretreatment for antigen retrieval is recommended. Suggested positive control: Human lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7min. at 700Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS15. Counterstain with Mayer's hemalum For further information and details see technicalinformation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 containig 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.1 % Kathon as a preservative.

Konservierungsmittel: Kathon CG

Vorsichtsmaßnahmen: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Andere Bezeichnung: ACE / CD143

Hintergrund: ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.Synonyms: Angiotensin-converting enzyme, DCP, DCP1, Dipeptidyl carboxypeptidase I

Gen-ID: 1636

NCBI Accession: NP_000780

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration