CD14 Antikörper (Biotin)

Produktnummer ABIN111908

Der Maus Monoklonal Anti-CD14-Antikörper wurde für FACS und IHC (fro) validiert. Er ist geeignet, CD14 in Proben von Human zu detektieren.

Kurzübersicht für CD14 Antikörper (Biotin) (ABIN111908)

Target: CD14

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: X-8

Produktdetails anti-CD14 Antikörper (Biotin)

Aufreinigung: Affinity chromatography.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 μg/mL,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15 % positiveHL-60 stimulated with DMSO 15 % positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 5 mg/mL bovine serum albumin (BSA) as a stabilizer without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. This product should be stored undiluted.

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gen-ID: 9606

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades