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CD11b Antikörper (FITC)

ITGAM Reaktivität: Human FACS, IF Wirt: Maus Monoclonal DCIS1-18 FITC
Produktnummer ABIN1112046
  • Target Alle CD11b (ITGAM) Antikörper anzeigen
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Reaktivität
    • 279
    • 179
    • 70
    • 33
    • 17
    • 17
    • 16
    • 14
    • 11
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 1
    Human
    Wirt
    • 154
    • 114
    • 105
    • 4
    • 1
    • 1
    Maus
    Klonalität
    • 279
    • 96
    • 1
    Monoklonal
    Konjugat
    • 126
    • 36
    • 32
    • 28
    • 21
    • 11
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD11b Antikörper ist konjugiert mit FITC
    Applikation
    • 279
    • 88
    • 79
    • 79
    • 62
    • 44
    • 38
    • 27
    • 24
    • 20
    • 13
    • 13
    • 12
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Produktmerkmale
    Monoclonal Mouse Anti-Human CD11b FITC.
    Klon
    DCIS1-18
    Isotyp
    IgG2a
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  • Applikationshinweise
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    Kommentare

    Fluorescein isothiocyanate (Molecular Probes).

    Probennahme
    1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD11b FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Lagerung
    4 °C
  • Sanchez-Madrid, Nagy, Robbins, Simon, Springer et al.: "A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), ..." in: The Journal of experimental medicine, Vol. 158, Issue 6, pp. 1785-1803, (1984) (PubMed).

  • Target
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Andere Bezeichnung
    CD11b (ITGAM Produkte)
    Synonyme
    CD11b/CD18 antikoerper, CR3 antikoerper, CR3A antikoerper, Cd11b antikoerper, F730045J24Rik antikoerper, Ly-40 antikoerper, MAC1 antikoerper, Mac-1 antikoerper, Mac-1a antikoerper, CD11B antikoerper, MAC-1 antikoerper, MAC1A antikoerper, MO1A antikoerper, SLEB6 antikoerper, C3bi antikoerper, integrin subunit alpha M antikoerper, integrin alpha M antikoerper, integrin, alpha M (complement component 3 receptor 3 subunit) antikoerper, integrin alpha-M antikoerper, ITGAM antikoerper, Itgam antikoerper, LOC100351865 antikoerper
    Hintergrund
    Integrin heterodimer of MW 165,95kDa which comprises the receptor (CR3 am chain, CD11b/ CD18) for the complement component C3i. The CR3 receptor is expressed as a surface molecule by circulating monocytes and granulocytes and also by dendritic reticulum cells in sections of lympoid tissue. Expression is increased on activated granulocytes.
    Pathways
    Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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