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AKT2 Antikörper (Internal Region)

Der Ratte Monoklonal Anti-AKT2-Antikörper wurde für WB, IHC, ELISA, FACS und IP validiert. Er ist geeignet, AKT2 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.
Rockland
Produktnummer ABIN1043774
Hersteller Produkt- Nr.: 200-501-e71

Kurzübersicht für AKT2 Antikörper (Internal Region) (ABIN1043774)

Target

Alle AKT2 Antikörper anzeigen
AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reaktivität

  • 168
  • 94
  • 61
  • 11
  • 8
  • 8
  • 7
  • 7
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
Human

Wirt

  • 149
  • 27
  • 6
  • 2
  • 1
  • 1
Ratte

Klonalität

  • 152
  • 32
Monoklonal

Konjugat

  • 101
  • 11
  • 11
  • 10
  • 8
  • 8
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Dieser AKT2 Antikörper ist unkonjugiert

Applikation

  • 156
  • 88
  • 71
  • 33
  • 28
  • 26
  • 26
  • 21
  • 19
  • 11
  • 8
  • 7
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon

16G11-E8
  • Bindungsspezifität

    • 21
    • 17
    • 15
    • 15
    • 15
    • 11
    • 8
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Hersteller Produkt- Nr.

    200-501-e71

    Hersteller

    Rockland

    Verwendungszweck

    AKT2 Antibody

    Kreuzreaktivität (Details)

    Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein.

    Produktmerkmale

    Synonyms: rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

    Western Blot Dilution: 1:500- 1:2000

    Immunoprecipitation Dilution: 20 μg/mL

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Altomare, Kozak, Sonoda, Testa: "Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene." in: Cytogenetics and cell genetics, Vol. 74, Issue 4, pp. 248-51, (1997) (PubMed).

  • Target

    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

    Andere Bezeichnung

    AKT2

    Hintergrund

    Background: AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

    Gen-ID

    208

    NCBI Accession

    NP_001317440

    UniProt

    P31751

    Pathways

    PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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