anti-Affe P2RX7 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended P2RX7 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) Antikörper
  • p2xr7
  • P2RX7
  • P2X7
  • AI467586
  • P2X(7)
  • P2X7R
  • p2x7
  • purinergic receptor P2X 7
  • purinergic receptor P2X, ligand-gated ion channel, 7
  • P2X purinoceptor 7
  • P2RX7
  • p2rx7
  • LOC100458463
  • P2rx7
Epitop
Internal Region
21
18
14
4
4
3
3
2
2
2
2
2
2
1
1
1
1
Reaktivität
Human, Affe
93
78
39
5
4
4
4
3
3
2
1
Wirt
Kaninchen
77
25
19
2
Klonalität
Polyklonal
Konjugat
Dieser P2RX7 Antikörper ist unkonjugiert
8
4
4
4
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
77
50
41
26
23
20
18
13
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1049197
$ 451.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1737743 IHC (p) Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) Antikörper
Epitop Internal Region
(21), (18), (14), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Human, Affe
(93), (78), (39), (5), (4), (4), (4), (3), (3), (2), (1)
Wirt Kaninchen
(77), (25), (19), (2)
Klonalität
Polyklonal
Konjugat Dieser P2RX7 Antikörper ist unkonjugiert
(8), (4), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(77), (50), (41), (26), (23), (20), (18), (13), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-P2RX7 Antikörper

Target Details P2RX7 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human P2RX7 / P2X7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX59 (56 % ).
Homologie Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%) Marmoset, Pig (88%) Mouse, Horse (81%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human P2RX7 / P2X7. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%), Marmoset, Pig (88%), Mouse, Horse (81%).

Type of Immunogen: Synthetic peptide

Target Details P2RX7

Produktdetails anti-P2RX7 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung P2RX7 / P2X7 (P2RX7 Antibody Abstract)
Hintergrund Name/Gene ID: P2RX7
Subfamily: P2X receptor
Family: Ion Channel

Synonyms: P2RX7, ATP receptor, p2X purinoceptor 7, p2X receptor 7, p2X7 purinoceptor, p2Z receptor, Purinergic receptor, p2RX7, p2X7, p2X7 receptor
Gen-ID 5027
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis

Anwendungsinformationen

Produktdetails anti-P2RX7 Antikörper Target Details P2RX7 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 - 20 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-P2RX7 Antikörper Target Details P2RX7 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-P2RX7 Antikörper Target Details P2RX7 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (Internal Region) antibody (ABIN1049197) Anti-P2RX7 / P2X7 antibody ABIN1049197 IHC staining of human pancreas, nerve. Immuno...