anti-Human Spectrin, Beta, Non-erythrocytic 2 Antikörper für Immunohistochemistry

Recommended Spectrin, Beta, Non-erythrocytic 2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) Antikörper
  • Spnb3
  • mKIAA0302
  • GTRAP41
  • SCA5
  • spectrin beta, non-erythrocytic 2
  • spectrin, beta, non-erythrocytic 2
  • Sptbn2
  • SPTBN2
Epitop
AA 2150-2200
31
15
10
8
3
2
1
1
Reaktivität
Human
188
71
52
1
Wirt
Kaninchen
134
55
Klonalität
Polyklonal
Konjugat
Unkonjugiert
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
4
4
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
146
142
137
131
110
64
18
15
14
8
Hersteller
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Bilder

Produktnummer ABIN189713
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
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9.452184 ABIN4355620 IHC Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4355597 FACS ICC IF IHC IHC (p) WB Mouse IgG3 kappa Anmelden zum Anzeigen 3B1NB
1 ABIN4355599 FACS ICC IF IHC IHC (p) WB Mouse IgG3 kappa Anmelden zum Anzeigen 3B1NB
1 ABIN4355619 ICC IF IHC IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4355596 FACS ICC IF IHC IHC (p) WB Mouse IgG3 kappa Anmelden zum Anzeigen 3B1NB
1 ABIN4245194 IHC IP Biotin Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal
1 ABIN4355582 IHC IHC (p) WB DyLight 650 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355595 IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355581 IHC IHC (p) WB Biotin Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355591 IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355592 ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal
1 ABIN4355583 IHC IHC (p) WB DyLight 488 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355584 IHC IHC (p) WB DyLight 550 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355585 IHC IP DyLight 650 Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal
1 ABIN4355586 IHC IP DyLight 488 Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal
1 ABIN4355587 IHC IP DyLight 550 Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal
1 ABIN4355589 IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 2340-2390, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4355590 ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Rabbit AA 2150-2200 Anmelden zum Anzeigen Polyclonal

General

Antigen Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) Antikörper
Epitop AA 2150-2200
(31), (15), (10), (8), (3), (2), (1), (1)
Reaktivität Human
(188), (71), (52), (1)
Wirt Kaninchen
(134), (55)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(146), (142), (137), (131), (110), (64), (18), (15), (14), (8)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Reinigung Immunogen affinity purified
Immunogen The epitope recognized by this antibody maps to a region between residues 2150 and 2200 of Spectrin beta 3 using the numbering given in entry NP_008877.1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Spectrin beta 3 (SPTBN2 Antibody Abstract)
Hintergrund Gene Symbol: SPTBN2
Molekulargewicht Theoretical MW: 271 kDa
Gen-ID 6712
UniProt O15020
Pathways Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:250, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-ParaffinThis Spectrin beta 3 antibody is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections. It is not recommended for Western blot analysis. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Not for use in: Western Blot
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll ICC/IF protocol specific for Spectrin beta 3 antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 2150-2200) antibody (ABIN189713) anti-Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 2150-2200) antibody
Immunohistochemistry (IHC) image for anti-Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 2150-2200) antibody (ABIN189713) Detection of Spectrin Beta-III in human hepatocytes using ABIN189713.
Immunofluorescence (IF) image for anti-Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 2150-2200) antibody (ABIN189713) Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [ABIN1897133] - Spec...