HRAS Protein (AA 1-186) (His tag)

Produktnummer ABIN666969

Dieses Recombinant HRAS-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für HRAS Protein (AA 1-186) (His tag) (ABIN666969)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-186

Aufreinigungstag / Konjugat: Dieses HRAS Protein ist gelabelt mit His tag.

Sequenz: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKC

Produktmerkmale: HRAS, 1-186aa, Human, His-tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Andere Bezeichnung: H-Ras

Hintergrund: HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 22.0 kDa (194aa) confirmed by MALDI-TOF

NCBI Accession: NP_001123914

Pathways: p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling