IL12A & IL27B (AA 20-189) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949030, Anbieter: Anmelden zum Anzeigen
Proteinname
Proteineigenschaft
AA 20-189
6
4
3
3
3
1
1
1
Spezies
Human
8
8
1
Quelle
HEK-293 Cells
7
6
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
AVI tag,Biotin,His tag
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Molekulargewicht 21.9 kDa (IL23A) & 34.7 kDa (IL12B)
NCBI Accession NP_057668
UniProt Q9NPF7, P29460
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for IL12A & IL27B (AA 20-189) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949030) Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer on SDS-PAGE under reducing (R...
Binding Studies (Bind) image for IL12A & IL27B (AA 20-189) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949030) Immobilized Anti-IL23A mAb, Human IgG1 at 2μg/mL (100 μL/well) can bind Biotinylated ...
Binding Studies (Bind) image for IL12A & IL27B (AA 20-189) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949030) Immobilized Anti-IL23A&IL12B(P40 domain) mAb, Human IgG1 at 2μg/mL (100 μL/well) can ...
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