TNFRSF11A Protein (AA 29-213, C-Term)

Produktnummer ABIN2666569

Produktdetails

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 29-213, C-Term

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Western Blotting (WB)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 50 - 200 ng/mL, as determined by inhibition of osteoclast differentiation induced by RANKL (20 ng/mL) on RAW246.7 macrophages.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Andere Bezeichnung: RANK (TNFRSF11A Produkte)

Synonyme: RANK Protein, RGD1563614 Protein, TNFRSF11A Protein, Ly109 Protein, ODFR Protein, OFE Protein, Rank Protein, TRANCE-R Protein, mRANK Protein, CD265 Protein, FEO Protein, LOH18CR1 Protein, OPTB7 Protein, OSTS Protein, PDB2 Protein, TRANCER Protein, TNF receptor superfamily member 11A Protein, TNF receptor superfamily member 11a Protein, tumor necrosis factor receptor superfamily, member 11a, NFKB activator Protein, Tnfrsf11a Protein, TNFRSF11A Protein

Hintergrund: RANK was initially cloned from dendritic cells. It is a type I membrane 66 kD protein that is expressed in monocytes, macrophages, dendritic cells, osteoclast precursors, and among other cells. RANK and its ligand (RANKL) play a key role in bone homeostasis, organogenesis, immune tolerance, and cancer. RANK and RANKL are members of the TNF family and are respectively designated as TNFRSF11A and TNFSF11. RANK, as well as other TNF receptor members, lacks intrinsic enzymatic activity. Its signal transduction is carried out by the recruitment of various adaptor proteins, including TNFR-associated factors (TRAFs), leading to the activation of JNK, ERK, p38, NFATc1, AKT, and NF-κB signaling pathways. In addition, INF-γ, IFN-β, and ITAM-activated costimulatory signals crosstalk with RANK signaling to regulate osteoclastogenesis in the opposite manner. While IFN-γ and IFN-β inhibit, ITAM stimulates this process. RANK and its ligand are associated to bone diseases such as postmenopausal osteoporosis and bone loss in inflammatory conditions. RANKL expressed on activated T-cells induces bone loss and joint destruction in rheumatoid arthritis. Also, RANK provides signals during medullary thymic epithelial cells (mTEC) development. mTECs contribute to the selection and survival of immunosuppressive Foxp3-positive regulatory T cells.

Molekulargewicht: The 191 amino acid recombinant protein has a predicted molecular mass of approximately 47.8 kDa. The protein migrates at approximately 60 kDa in DTT-reduced conditions and at approximately 120 kDa in non-reduced conditions by SDS-PAGE. The predicted N-ter

Pathways: NF-kappaB Signalweg