anti-Maus MTOR Antikörper für Immunofluorescence

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
AA 185-290
66
36
28
23
21
15
15
13
12
10
8
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Human, Maus, Ratte (Rattus)
378
200
197
18
9
8
7
7
7
6
4
2
2
2
1
1
Wirt
Maus
325
48
4
1
Klonalität
Monoklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Immunofluorescence (IF), Western Blotting (WB)
252
114
99
75
56
52
37
22
8
7
7
7
3
2
2
2
2
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN968383
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.14905 ABIN1531447 IF IHC ELISA Rabbit IgG AA 2447-2496, pSer2481 Anmelden zum Anzeigen Polyclonal
12.14905 ABIN2705013 IC IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
12.14905 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN968384 IF WB Mouse IgG1 AA 185-290 Anmelden zum Anzeigen 30-RAFT1 5
1 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 1
1 ABIN196962 IF IHC (p) WB Rabbit pSer2448 Anmelden zum Anzeigen Polyclonal 4
1 ABIN319329 IF IHC (p) WB Rabbit Ser2448 Anmelden zum Anzeigen Polyclonal 3
1 ABIN2879300 IF IHC (p) WB Rabbit pSer2442 Anmelden zum Anzeigen Polyclonal
1 ABIN362262 IF IHC Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal 3
1 ABIN362868 IF IHC WB Rabbit IgG AA 2446-2450 Anmelden zum Anzeigen Polyclonal 1
1 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal
1 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal
1 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Anmelden zum Anzeigen Polyclonal
1 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Anmelden zum Anzeigen Polyclonal
1 ABIN5555365 EIA IF WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1737123 IF IHC (p) ELISA WB Rabbit IgG AA 2440-2457 Anmelden zum Anzeigen Polyclonal
1 ABIN498610 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN302811 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN214561 IF IHC (p) ELISA WB Rabbit AA 2440-2457 Anmelden zum Anzeigen Polyclonal
1 ABIN1822694 IF IP WB Rabbit IgG pSer2448 Anmelden zum Anzeigen

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop AA 185-290
(66), (36), (28), (23), (21), (15), (15), (13), (12), (10), (8), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Huhn, Human, Maus, Ratte (Rattus)
(378), (200), (197), (18), (9), (8), (7), (7), (7), (6), (4), (2), (2), (2), (1), (1)
Wirt Maus
(325), (48), (4), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser MTOR Antikörper ist unkonjugiert
(8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Applikation Immunofluorescence (IF), Western Blotting (WB)
(252), (114), (99), (75), (56), (52), (37), (22), (8), (7), (7), (7), (3), (2), (2), (2), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MTOR Antikörper

Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN968383) Bilder
Kreuzreaktivität Maus, Human, Huhn
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat RAFT1 aa. 185-290
Klon 30-RAFT1
Isotyp IgG1

Target Details MTOR

Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN968383) Bilder zurück nach oben
Antigen
Andere Bezeichnung RAFT1 (FRAP1 Antibody Abstract)
Hintergrund Immunosuppressive agents such as cyclosporin A, FK506, and rapamycin are used routinely in clinical transplantation and the treatment of autoimmune disorders. Cyclosporin A and FK506 bind to cyclophilins and the FK-binding protein FKBP12, respectively. These complexes interact with, and inhibit the activity of, the calcineurin protein phosphatase, which is essential for T cell activation. Rapamycin also interacts with FKBP12, but this complex does not affect calcineurin. Instead, it inhibits signals required for G1 to S progression in yeast, in IL-2-stimulated T lymphocytes, and other mammalian cell types. Rapamycin-FKBP12 binds to a protein designated Rapamycin And FKBP12 Target-1 (RAFT1) in rats and FKBP-Rapamycin-Associated Protein (FRAP) in humans. RAFT1 is the homolog of the yeast genes TOR1 and TOR2 which, when mutated, lead to rapamycin resistance. RAFT1 is a PI4-kinase that exhibits homology with other PI kinases such as PI3-kinase and PI4-Kalpha. RAFT1 phosphorylates p70 S6 kinase and 4E-BP1 which are regulators of translational initiation. However, rapamycin-FKBP1 does not inhibit PI4-kinase activity and, therefore, affects RAFT1 activity via alternate mechanisms.
Synonyms: Rapamycin And FKBP12 Target-1
Molekulargewicht 245 kDa
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Referenzen für anti-MTOR Antikörper (ABIN968383) Bilder zurück nach oben
Kommentare

Related Products: ABIN968539, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Referenzen für anti-MTOR Antikörper (ABIN968383) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-MTOR Antikörper (ABIN968383)

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Castedo, Ferri, Blanco, Roumier, Larochette, Barretina, Amendola, Nardacci, Métivier, Este, Piacentini, Kroemer: "Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation." in: The Journal of experimental medicine, Vol. 194, Issue 8, pp. 1097-110, 2001

Alarcon, Cardenas, Heitman: "Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast." in: Genes & development, Vol. 10, Issue 3, pp. 279-88, 1996

Sabatini, Pierchala, Barrow, Schell, Snyder: "The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20875-8, 1995

Allgemeine Veröffentlichungen

Burnett, Barrow, Cohen, Snyder, Sabatini: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998

Sabatini, Erdjument-Bromage, Lui, Tempst, Snyder: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994

Bilder

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN968383) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 185-290) antibody (ABIN968383) Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-172...
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 185-290) antibody (ABIN968383) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).