anti-Hund MTOR Antikörper für Western Blotting

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
AA 2440-2457
66
36
28
23
21
15
15
13
11
10
8
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Zebrafisch (Danio rerio)
378
200
198
18
8
8
7
7
7
7
4
2
2
1
1
1
Wirt
Kaninchen
324
49
4
1
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
252
114
99
75
57
52
37
22
7
7
7
7
3
2
2
2
2
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN401320
$ 419.10
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Anmelden zum Anzeigen Polyclonal
1 ABIN328662 ELISA WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal
1 ABIN601928 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1915270 IP WB Rabbit IgG AA 1675-1725 Anmelden zum Anzeigen Polyclonal
1 ABIN1915273 WB Rabbit IgG AA 200-250 Anmelden zum Anzeigen Polyclonal

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop AA 2440-2457
(66), (36), (28), (23), (21), (15), (15), (13), (11), (10), (8), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Zebrafisch (Danio rerio)
(378), (200), (198), (18), (8), (8), (7), (7), (7), (7), (4), (2), (2), (1), (1), (1)
Wirt Kaninchen
(324), (49), (4), (1)
Klonalität
Polyklonal
Konjugat Dieser MTOR Antikörper ist unkonjugiert
(8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
(252), (114), (99), (75), (57), (52), (37), (22), (7), (7), (7), (7), (3), (2), (2), (2), (2), (1), (1)
Pubmed 6 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MTOR Antikörper

Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN401320) Bilder
Spezifität Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from derived tissues and cells.
Kreuzreaktivität (Details) Species reactivity (expected):Rat, Mouse, Zebrafish, Canine.
Species reactivity (tested):Human.
Reinigung Immunoaffinity chromatography
Immunogen Synthetic peptide corresponding to amino acids 2440-2457 of human mTOR
Isotyp IgG

Target Details MTOR

Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN401320) Bilder zurück nach oben
Antigen
Andere Bezeichnung mTOR / FRAP1 (FRAP1 Antibody Abstract)
Hintergrund Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12 - rapamycin associated protein 1, FK506 binding protein12 - rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gen-ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Referenzen für anti-MTOR Antikörper (ABIN401320) Bilder zurück nach oben
Applikationshinweise ELISA: 1/4000 - 1/20000. Western Blot: 1/250 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Referenzen für anti-MTOR Antikörper (ABIN401320) Bilder zurück nach oben
Konzentration 0.40 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C

Referenzen für anti-MTOR Antikörper (ABIN401320)

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003

McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002

Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002

Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002

Bilder

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN401320) zurück nach oben
Bilder des Herstellers
 image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody
 image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (Image 2)