anti-Maus Tumor Necrosis Factor Antikörper für Immunoprecipitation

Recommended Tumor Necrosis Factor Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (TNF) Antikörper
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reaktivität
Maus
1259
464
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Wirt
Kaninchen
942
520
186
49
10
9
7
6
4
3
2
Klonalität
Polyklonal
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
169
100
78
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
8
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
888
859
633
322
287
229
221
191
139
117
96
68
30
28
26
22
20
18
14
9
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Hersteller
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Produktnummer ABIN118027
$ 1,204.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1582139 IP IHC ELISA WB Mouse IgG Anmelden zum Anzeigen 2
1 ABIN2661299 CyTOF FACS IF IHC IP Biotin Goat IgG Anmelden zum Anzeigen Polyclonal 3
1 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Anmelden zum Anzeigen 52B83 4
1 ABIN967569 IP Neut ELISA WB Armenian Hamster IgG1, lambda Anmelden zum Anzeigen TN3-19-12 6
1 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Anmelden zum Anzeigen TN3 6
1 ABIN1875124 IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN118025 EIA Func IHC (fro) IP RIA WB Rabbit Anmelden zum Anzeigen Polyclonal 2
1 ABIN4360976 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 488 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360982 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360985 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 755 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360986 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360989 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360990 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360977 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360975 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360978 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360981 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360988 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN4360983 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Anmelden zum Anzeigen SPM543
1 ABIN265631 BP ELISA IP Neut WB Armenian Hamster IgG2a Anmelden zum Anzeigen TN3-19-12

General

Antigen Tumor Necrosis Factor (TNF) Antikörper
Reaktivität Maus
(1259), (464), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Wirt Kaninchen
(942), (520), (186), (49), (10), (9), (7), (6), (4), (3), (2)
Klonalität
Polyklonal
Konjugat Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
(169), (100), (78), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(888), (859), (633), (322), (287), (229), (221), (191), (139), (117), (96), (68), (30), (28), (26), (22), (20), (18), (14), (9), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Tumor Necrosis Factor Antikörper

Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN118027) Bilder
Kreuzreaktivität (Details) Species reactivity (tested):Mouse.
Reinigung Selective salt precipitation
Immunogen Recombinant Mouse TNF-alpha produced in E.coli.

Target Details Tumor Necrosis Factor

Produktdetails anti-Tumor Necrosis Factor Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN118027) Bilder zurück nach oben
Antigen
Andere Bezeichnung Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Hintergrund Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gen-ID 10090
UniProt P06804
Forschungsgebiet Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN118027) Bilder zurück nach oben
Applikationshinweise Western Blot: Use HRP Goat-anti-Rabbit IgG [H&L] (code R1364HRP) and TMB as asubstrate. A working dilution range of 1: 500 to 1: 2,000 is suggested for this application to detect TNFαfrom supernatants or lysates of 2 x 10^6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN118027) Bilder zurück nach oben
Konzentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 2-8 °C.

Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN118027)

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Birkas Kovats, Palik, Faludi, Cseh: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006

Nedwin, Naylor, Sakaguchi, Smith, Jarrett-Nedwin, Pennica, Goeddel, Gray: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985