anti-Maus Tumor Necrosis Factor Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended Tumor Necrosis Factor Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (TNF) Antikörper
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitop
AA 180-230
52
44
33
27
24
22
19
17
14
11
11
10
10
8
8
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
1258
464
358
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Wirt
Kaninchen
942
520
186
49
10
9
7
6
4
3
2
Klonalität
Polyklonal
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
169
100
78
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
8
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
888
859
633
322
287
230
221
191
139
118
97
69
30
29
25
22
20
18
14
9
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN677318
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN677323 IF (p) Cy5.5 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN677321 IF (p) Cy3 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN677322 IF (p) Cy5 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN677324 IF (p) Cy7 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN677325 IF (p) FITC Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN918042 IF (p) Alexa Fluor 555 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN918031 IF (p) Alexa Fluor 647 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN918032 IF (p) Alexa Fluor 350 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN918034 IF (p) Alexa Fluor 488 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN2807091 IF (p) Alexa Fluor 594 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN5011738 IF (p) Alexa Fluor 680 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal
1 ABIN5011739 IF (p) Alexa Fluor 750 Rabbit IgG AA 180-230 Anmelden zum Anzeigen Polyclonal

General

Antigen Tumor Necrosis Factor (TNF) Antikörper
Epitop AA 180-230
(52), (44), (33), (27), (24), (22), (19), (17), (14), (11), (11), (10), (10), (8), (8), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(1258), (464), (358), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Wirt Kaninchen
(942), (520), (186), (49), (10), (9), (7), (6), (4), (3), (2)
Klonalität
Polyklonal
Konjugat Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
(169), (100), (78), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(888), (859), (633), (322), (287), (230), (221), (191), (139), (118), (97), (69), (30), (29), (25), (22), (20), (18), (14), (9), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Tumor Necrosis Factor Antikörper

Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotyp IgG

Target Details Tumor Necrosis Factor

Produktdetails anti-Tumor Necrosis Factor Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318) Bilder zurück nach oben
Antigen
Andere Bezeichnung Tnf alpha (TNF Antibody Abstract)
Hintergrund

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

Gen-ID 21926
UniProt P06804
Forschungsgebiet Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318)

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." in: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Mouse (Murine)).

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, 2015

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, 2015

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." in: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013

Bilder

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN677318) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Antigen: 0.2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300...
'Independent Validation' Siegel
Chargennummer 140113
Validierte Anwendung Immunofluorescence
Positivkontrolle Normal mouse lung tissue
Negativkontrolle Normal mouse adipose tissue
Bewertung Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protokoll
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Anmerkungen Nothing to note.
Validierungsbilder
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only