SERPINB6 ELISA Kit

Produktnummer ABIN5657845

Produktdetails SERPINB6 ELISA Kit

Target: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Placental Thrombin Inhibitor (PTI). No significant cross-reactivity or interference between Placental Thrombin Inhibitor (PTI) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placental Thrombin Inhibitor (PTI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placental Thrombin Inhibitor (PTI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placental Thrombin Inhibitor (PTI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placental Thrombin Inhibitor (PTI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SERPINB6

Target: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Andere Bezeichnung: Placental Thrombin Inhibitor (SERPINB6 Produkte)

Synonyme: Serpinb6a ELISA Kit, cap ELISA Kit, dfnb91 ELISA Kit, mstp057 ELISA Kit, pi6 ELISA Kit, pti ELISA Kit, serpinb8 ELISA Kit, spi3 ELISA Kit, 4930482L21Rik ELISA Kit, D330015H01Rik ELISA Kit, PI-6 ELISA Kit, Serpinb6 ELISA Kit, Spi3 ELISA Kit, ovalbumin ELISA Kit, CAP ELISA Kit, DFNB91 ELISA Kit, MSTP057 ELISA Kit, PI6 ELISA Kit, PTI ELISA Kit, SPI3 ELISA Kit, serpin family B member 6A ELISA Kit, serpin family B member 6 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6a ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 6 ELISA Kit, Serpinb6a ELISA Kit, serpinb6 ELISA Kit, SERPINB6 ELISA Kit

Hintergrund:

Gene Name: Placental Thrombin Inhibitor

Gene Aliases: SERPINB6, CAP, PI6, SPI3, Serpin Peptidase Inhibitor Clade B Member 6, Cytoplasmic antiproteinase, Peptidase inhibitor 6

Gen-ID: 5269

UniProt: P35237