anti-Affe SOCS3 Antikörper für Western Blotting

Recommended SOCS3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Suppressor of Cytokine Signaling 3 (SOCS3) Antikörper
  • socs3
  • fj09e12
  • zgc:56537
  • wu:fj09e12
  • MGC64393
  • MGC75749
  • xsocs3
  • MGC81248
  • atod4
  • cis3
  • cish3
  • socs-3
  • ssi-3
  • ssi3
  • SOCS3
  • ATOD4
  • CIS3
  • Cish3
  • SOCS-3
  • SSI-3
  • SSI3
  • Cis3
  • EF-10
  • Ef10
  • Ssi3
  • Socs-3
  • Ssi-3
  • suppressor of cytokine signaling 3a
  • suppressor of cytokine signaling 3
  • socs3a
  • socs3
  • SOCS3
  • LOC100227853
  • Socs3
Epitop
AA 202-225
30
21
9
8
4
4
4
3
3
2
2
1
1
1
1
1
Reaktivität
Human, Affe, Maus, Ratte (Rattus)
203
70
40
8
8
6
4
4
2
2
2
2
2
1
1
1
Wirt
Kaninchen
124
82
16
Klonalität
Polyklonal
Konjugat
Dieser SOCS3 Antikörper ist unkonjugiert
10
7
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Western Blotting (WB)
187
68
56
37
32
25
7
6
5
2
2
1
1
1
1
Hersteller
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Produktnummer ABIN493472
462,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN293840 WB Goat AA 213-225 Anmelden zum Anzeigen Polyclonal
1 ABIN2628599 IHC (p) WB Rabbit IgG AA 71-85 Anmelden zum Anzeigen Polyclonal

anti-SOCS3 Antikörper für Western Blotting mit den meisten Publikationen

Ähnliche anti-SOCS3 Antikörper

Applikation / Reaktivität Human Maus Ratte (Rattus) Affe
Immunofluorescence (fixed cells) (IF/ICC) 3 Antikörper 2 Antikörper 2 Antikörper
Western Blotting (WB) 170 Antikörper 65 Antikörper 35 Antikörper 3 Antikörper
Immunoprecipitation (IP) 5 Antikörper 4 Antikörper 4 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antikörper 5 Antikörper 3 Antikörper 1 Antikörper
Immunohistochemistry (IHC) 19 Antikörper 48 Antikörper 17 Antikörper

General

Antigen Suppressor of Cytokine Signaling 3 (SOCS3) Antikörper
Epitop AA 202-225
(30), (21), (9), (8), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Affe, Maus, Ratte (Rattus)
(203), (70), (40), (8), (8), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(124), (82), (16)
Klonalität
Polyklonal
Konjugat Dieser SOCS3 Antikörper ist unkonjugiert
(10), (7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Western Blotting (WB)
(187), (68), (56), (37), (32), (25), (7), (6), (5), (2), (2), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-SOCS3 Antikörper

Target Details SOCS3 Anwendungsinformationen Handhabung ProductDetails: References for anti-SOCS3 antibody (ABIN493472) Bilder
Sequenz DSY EKV TQL PGP IRE FLD QYD APL
Spezifität This antibody labels a broad band at 27-29 kDa in SOCS3 western blot control samples. The antibody also reacts to a 29 kDa protein in Rat pancreas extracts.
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse, Rat and Monkey.
Reinigung Affinity Chromatography on immobilized antigen
Immunogen Synthetic peptide corresponding to amino acids 202-225 of the Human Repressor of cytokine signaling 3 protein. The peptide was amidated and conjugated to a carrier protein
Isotyp IgG

Target Details SOCS3

Produktdetails anti-SOCS3 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-SOCS3 antibody (ABIN493472) Bilder zurück nach oben
Antigen
Andere Bezeichnung SOCS3 / CIS3 (SOCS3 Antibody Abstract)
Hintergrund Suppressors of cytokine signaling (SOCS) proteins are intracellular regulators of receptor signal transduction, mainly Janus kinase/signal transducers and activators of transcription (JAK/STAT). The suppressor for cytokine signaling 3 is a key regulator of cytokine signaling in inflammatory diseases and in breast cancer progression. Over expression of SOCS3 in response to prolactin, suppresses STAT3 expression and abrogate STAT5 phosphorylation which resulted in decrease cell proliferation of T47D breast cancer cells. SOCS3 acts as an anti-proliferative agent in breast cancer cells whose mechanism of action and cross talk with other cellular pathways are complex (1). The IL6 signaling on both hepatic and hematopoietic cells is mediated through gp130 receptor and SOCS3 is a critical inhibitor of this pathway (2). SOCS3 knockout mice revealed significant enhancement in DNA replication and liver weight restoration after partial hepatectomy (PH) compared to littermate controls. The absence of SOCS3, STAT3 phosphorylation is prolonged activation of ERK1/2 is enhances after PH. SOCS protein inhibit cytokine signaling by targeting different component of signaling complex. These include either direct binding of SOCS to activated , tyrosine phosphorylated cytokine receptors ot JAK via their Src homology 2 doamin or via targeting receptor complexes for proteosomal degradation via SOC box (3). SOCS3 has been shown to regulate the activity of proline rich tyrosine kinase 2 (PYK2) in A549 cells as a result of epigenetic regulatory mechanism involving methylation of exon 2, and 5. Recent literature suggest that infections (hepatitis C and Leishmania donovani) caused an induction is SOCS3 that interfere with host immune responses. There are eight SOCS family members known, SOCS1, SOCS2 and SOCS3, SOCS2 can enhance IL2 and IL3 induced STAT phosphorylation followed bt potentiate proliferation in response to cytokine stimulation. Expression of SOCS2 also resulted in marked proteosome-dependent reduction in SOCS3 and SOCS1 proteins. SOCS2 also bind to SOCS3 and elongin B/C to form an E3 ligase complex resulting in degradation of SOCS3 (4). SOCS3 is localized on chromosome 17q25 and was found to be expressed at higher concentration in skin of atopic dermatitis patients than in healthy individuals (5). SOCS3 is a 225 amino acid containing protein with apparent MW 28 kDa. SOCS3 protein contains several phospho-tyrosine binding pockets at the N-terminal end, a SH2 superfamily domain and a SOCS-SOCS3 family domain near the C-terminal end called SOCS box.Synonyms: CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3, SSI-3, SSI3, STAT-induced STAT inhibitor 3, Suppressor of cytokine signaling 3
Molekulargewicht 29 kDa
Gen-ID 9021
NCBI Accession NP_003946
UniProt O14543
Pathways JAK-STAT Signalweg, Response to Growth Hormone Stimulus, Hepatitis C

Anwendungsinformationen

Produktdetails anti-SOCS3 Antikörper Target Details SOCS3 Handhabung ProductDetails: References for anti-SOCS3 antibody (ABIN493472) Bilder zurück nach oben
Applikationshinweise This antibody is ideal for Western Blot (> 1/500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-SOCS3 Antikörper Target Details SOCS3 Anwendungsinformationen ProductDetails: References for anti-SOCS3 antibody (ABIN493472) Bilder zurück nach oben
Konzentration 0.58-0.65 mg/mL
Buffer Stabilization buffer
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store the antibody undiluted (in aliquots) at-20 °C.

ProductDetails: References for anti-SOCS3 antibody (ABIN493472)

Produktdetails anti-SOCS3 Antikörper Target Details SOCS3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Barclay, Anderson, Waters, Curlewis: "SOCS3 as a tumor suppressor in breast cancer cells, and its regulation by PRL." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 8, pp. 1756-66, 2009

Ekelund, Saaf, Tengvall-Linder, Melen, Link, Barker, Reynolds, Meggitt, Kere, Wahlgren, Pershagen, Wickman, Nordenskjold, Kockum, Bradley: "Elevated expression and genetic association links the SOCS3 gene to atopic dermatitis." in: American journal of human genetics, Vol. 78, Issue 6, pp. 1060-5, 2006

Tannahill, Elliott, Barry, Hibbert, Cacalano, Johnston: "SOCS2 can enhance interleukin-2 (IL-2) and IL-3 signaling by accelerating SOCS3 degradation." in: Molecular and cellular biology, Vol. 25, Issue 20, pp. 9115-26, 2005

Alexander, Hilton: "The role of suppressors of cytokine signaling (SOCS) proteins in regulation of the immune response." in: Annual review of immunology, Vol. 22, pp. 503-29, 2004

Croker, Krebs, Zhang, Wormald, Willson, Stanley, Robb, Greenhalgh, Förster, Clausen, Nicola, Metcalf, Hilton, Roberts, Alexander: "SOCS3 negatively regulates IL-6 signaling in vivo." in: Nature immunology, Vol. 4, Issue 6, pp. 540-5, 2003