anti-Maus EPOR Antikörper für Immunocytochemistry

Recommended EPOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Erythropoietin Receptor (EPOR) Antikörper
  • EPOR
  • epor
  • EPO-R
  • erythropoietin receptor
  • Erythropoietin receptor
  • erythropoietin receptor homologue
  • erythropoietin receptor-like
  • EPOR
  • epor
  • Epor
  • LOC100355350
Epitop
AA 300-400, Internal Region
24
17
15
15
12
10
10
7
7
6
6
6
5
5
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
202
79
72
7
4
3
3
2
2
2
1
1
1
Wirt
Kaninchen
141
61
13
1
Klonalität
Polyklonal
Konjugat
Dieser EPOR Antikörper ist unkonjugiert
13
10
9
6
6
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
157
82
52
48
41
34
31
16
13
7
5
2
2
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN446737
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN4309592 ICC IF IHC IHC (p) SimWes WB Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN1172012 ICC IHC IP WB Rabbit IgG AA 45-244 Anmelden zum Anzeigen Polyclonal
1 ABIN4309595 ICC IF IHC IHC (p) WB Biotin Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309596 ICC IF IHC IHC (p) WB DyLight 350 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309597 ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309599 ICC IF IHC IHC (p) WB DyLight 488 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309601 ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309606 ICC IF IHC IHC (p) WB PerCP Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309608 ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309607 ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309593 ELISA ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4309603 ICC IF IHC IHC (p) WB DyLight 755 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309600 ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309602 ICC IF IHC IHC (p) WB DyLight 680 Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN342800 ICC IP IHC ELISA WB Sheep Extracellular Domain Anmelden zum Anzeigen Polyclonal
1 ABIN4309594 ICC IF IHC IHC (p) WB Allophycocyanin Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4309605 ICC IF IHC IHC (p) WB PE Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2857985 ICC IF WB Rabbit pTyr368 Anmelden zum Anzeigen Polyclonal
1 ABIN2858864 ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Erythropoietin Receptor (EPOR) Antikörper
Epitop AA 300-400, Internal Region
(24), (17), (15), (15), (12), (10), (10), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(202), (79), (72), (7), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(141), (61), (13), (1)
Klonalität
Polyklonal
Konjugat Dieser EPOR Antikörper ist unkonjugiert
(13), (10), (9), (6), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(157), (82), (52), (48), (41), (34), (31), (16), (13), (7), (5), (2), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-EPOR Antikörper

Target Details EPOR Anwendungsinformationen Handhabung Bilder
Spezifität EpoR (A364) detects endogenous levels of EpoR protein.
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]

Target Details EPOR

Produktdetails anti-EPOR Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Erythropoietin receptor (EPOR Antibody Abstract)
Hintergrund Gene Symbol: EPOR
Gen-ID 2057
UniProt P19235
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-EPOR Antikörper Target Details EPOR Handhabung Bilder zurück nach oben
Applikationshinweise Western Blot 2.0 μg/mL, Simple Western 1:10, Immunohistochemistry 1:50 - 1:100, Immunocytochemistry/Immunofluorescence 1:50 - 1:100, Immunohistochemistry-Paraffin 1:50-1:100In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western blot Protocol for EPO receptor antibody
Western Blot Protocol:
1. Perform SDS-PAGE on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute anti-HDAC5 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin protocol for EPO receptor antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-EPOR Antikörper Target Details EPOR Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bilder

Produktdetails anti-EPOR Antikörper Target Details EPOR Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Erythropoietin Receptor (EPOR) (AA 300-400), (Internal Region) antibody (ABIN446737) Immunocytochemistry/Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO ...
Western Blotting (WB) image for anti-Erythropoietin Receptor (EPOR) (AA 300-400), (Internal Region) antibody (ABIN446737) Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 ...
Simple Western (SimWes) image for anti-Erythropoietin Receptor (EPOR) (AA 300-400), (Internal Region) antibody (ABIN446737) Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view sho...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Erythropoietin Receptor (EPOR) (AA 300-400), (Internal Region) antibody (ABIN446737) Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis ...