Maus IgG isotype control (His tag)

Details zu Produkt Nr. ABIN1098612, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Maus
215
208
202
170
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Antikörpertyp
Recombinant
Konjugat
His tag
207
168
165
164
155
108
58
58
48
41
24
13
12
12
11
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Isotype Control (IsoC), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Reinigung purified by using conventional chromatography.
Reinheit > 90 % by SDS-PAGE
Hintergrund Immunoglobulin G (IgG) is antibody molecules. Each IgG is composed of four peptide chains - two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity(ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Synonyms: Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G
Molekulargewicht 27.5 kDa (242aa)
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Each Investigator should determine their own optimal working dilution for specific applications.
Kommentare

Concentration (detection method): 1 mg/mL (determined by Bradford assay)

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C
Bilder des Herstellers
 image for Mouse IgG isotype control (His tag) (ABIN1098612) Mouse IgG isotype control (His tag)
Allgemeine Veröffentlichungen Mallery, McEwan, Bidgood, Towers, Johnson, James: "Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 46, pp. 19985-90, 2010 (PubMed).

Stadlmann, Pabst, Kolarich, Kunert, Altmann: "Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides." in: Proteomics, Vol. 8, Issue 14, pp. 2858-71, 2008 (PubMed).