anti-Rind (Kuh) NCOA4 Antikörper für Western Blotting

Recommended NCOA4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Receptor Coactivator 4 (NCOA4) Antikörper
  • zgc:55307
  • zgc:77270
  • NCOA4
  • MGC138109
  • rfg
  • ele1
  • ptc3
  • ara70
  • MGC84996
  • DKFZp469A035
  • DKFZp459B2429
  • DKFZp469A1925
  • ncoa4
  • ARA70
  • ELE1
  • PTC3
  • RFG
  • 1110034E15Rik
  • 4432406M01Rik
  • AI227008
  • Rfg
  • nuclear receptor coactivator 4
  • nuclear receptor coactivator 4 S homeolog
  • Ncoa4
  • ncoa4
  • NCOA4
  • ncoa4.S
Epitop
N-Term
12
10
9
9
8
8
6
4
3
3
3
2
2
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus)
84
11
8
3
3
3
2
1
1
1
Wirt
Kaninchen
45
39
Klonalität
Polyklonal
Konjugat
Dieser NCOA4 Antikörper ist unkonjugiert
3
3
3
3
3
3
Applikation
Western Blotting (WB)
74
38
21
9
8
4
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2780237
$ 289.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.226648 ABIN2776441 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN798792 WB Rabbit AA 509-558 Anmelden zum Anzeigen Polyclonal
1 ABIN1976718 IP WB Rabbit IgG AA 564-614 Anmelden zum Anzeigen Polyclonal

General

Antigen Nuclear Receptor Coactivator 4 (NCOA4) Antikörper
Epitop N-Term
(12), (10), (9), (9), (8), (8), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus)
(84), (11), (8), (3), (3), (3), (2), (1), (1), (1)
Wirt Kaninchen
(45), (39)
Klonalität
Polyklonal
Konjugat Dieser NCOA4 Antikörper ist unkonjugiert
(3), (3), (3), (3), (3), (3)
Applikation Western Blotting (WB)
(74), (38), (21), (9), (8), (4), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-NCOA4 Antikörper

Target Details NCOA4 Anwendungsinformationen Handhabung Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN980536.
Sequenz CLIHQLEYTQ NKDLANQVSV CLERLGSLAL KPEDSTVLLF EADTSALRQT
Homologie Cow: 92%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Produktmerkmale This is a rabbit polyclonal antibody against Ncoa4. It was validated on Western Blot.
Reinigung Affinity Purified
Immunogen The immunogen is a synthetic peptide corresponding to a region of Mouse

Target Details NCOA4

Produktdetails anti-NCOA4 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Ncoa4 (NCOA4 Antibody Abstract)
Hintergrund The function of Ncoa4 remains unknown.
Alias Symbols: 1110034E15Rik, 4432406M01Rik, AI227008, ARA70, MGC103382, Rfg
Protein Interaction Partner: Ndn,
Protein Size: 625
Molekulargewicht 70 kDa
Gen-ID 27057
NCBI Accession NM_001033988, NP_001029160
Forschungsgebiet Chromatin and Nuclear Signaling
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway

Anwendungsinformationen

Produktdetails anti-NCOA4 Antikörper Target Details NCOA4 Handhabung Bilder zurück nach oben
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 625 AA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-NCOA4 Antikörper Target Details NCOA4 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Bilder

Produktdetails anti-NCOA4 Antikörper Target Details NCOA4 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Nuclear Receptor Coactivator 4 (NCOA4) (N-Term) antibody (ABIN2780237) anti-Nuclear Receptor Coactivator 4 (NCOA4) (N-Term) antibody
'Independent Validation' Siegel
Antigen Ncoa4
Chargennummer QC11327
Validierte Anwendung Western Blotting
Positivkontrolle HIF overexpression mice, WT mice
Negativkontrolle NCOA4-/- mice
Bewertung Passed, based on the fact that the antibody picked up the positive control (unpublished data suggests that NCOA4 is highly expressed in an intestinal HIF overexpression model).
Primärantikörper ABIN2780237
Sekundärantikörper Anti-rabbit HRP-linked secondary antibody (Cell Signaling, 7074S, lot 26)
Protokoll
  • Wild type (WT) or NCOA4 -/- mice were fed normal or iron-deficient diet for 7 days, followed by euthanization and scraping of duodenal epithelium (DE).
  • DE cells were lysed in RIPA buffer and whole cell lysate (WCL) prepared.
  • 50µg total protein from each sample were used for Western blot analysis.
  • Samples were denatured in 5x gel-loading buffer containing beta-marcaptoethanol and separated on a 10% SDS-PAGE gel (Laemmli 1970).
  • Transfer onto Nitrocellulose membrane (Biorad, 1620212, lot 10484060) was done on Trans-Blot SD apparatus (Bio-Rad) following the manufacturer’s protocol.
  • Membranes were blocked with TBS-tween (TBST) solution containing 5% milk for 1h.
  • Incubation with primary antibody overnight at 4°C:
    • NCOA4 antibody ABIN2780237 (lot QC11327) diluted 1:1000 in TBST containing 0.1% BSA.
    • FTH1 antibody (Cell signaling, 3998S; lot 1) diluted in TBST containing 0.1% BSA.
    • GAPDH antibody (Santa Cruz, SC-25778, lot D30115) diluted in TBST containing 0.1% BSA.
  • Washing with TBST for 15min at RT.
  • Incubation with anti-rabbit HRP-linked secondary antibody (Cell Signaling, 7074S, lot 26) diluted 1:2000 in TBST a shaker for 1h at RT.
  • Three rinses, followed by four 15min washes, twice TBST and twice with TBS.
  • Chemiluminescence detection was done using ECL Select Western blotting detection reagent (Amersham, RPN2235, lot 9723881).
  • Blot was developed on a Biorad imaging system.
Anmerkungen
  • The antibody did not consistently pick up the WT mice on normal and low iron diet.
  • Also, there is still a faint band in the NCOA4 -/- lane. We are confident that it is a true KO mouse, as there is high ferritin expression even after 7 days of low iron diet (compared to ferritin expression in WT low iron mice as well as that of the HIF over expression mouse, which are known to have high intestinal ftn content).
Validierungsbilder
Western Blotting validation image for anti-Nuclear Receptor Coactivator 4 (NCOA4) (N-Term) antibody (ABIN2780237) Mouse duodenum whole cell lysates from intestinal HIF overexpression (HIF OE) mice, N...