Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

NCOA4 Antikörper (N-Term)

NCOA4 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2780237
  • Target Alle NCOA4 Antikörper anzeigen
    NCOA4 (Nuclear Receptor Coactivator 4 (NCOA4))
    Bindungsspezifität
    • 9
    • 8
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 45
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen
    Wirt
    • 37
    • 8
    Kaninchen
    Klonalität
    • 37
    • 8
    Polyklonal
    Konjugat
    • 27
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NCOA4 Antikörper ist unkonjugiert
    Applikation
    • 32
    • 26
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Sequenz
    CLIHQLEYTQ NKDLANQVSV CLERLGSLAL KPEDSTVLLF EADTSALRQT
    Homologie
    Cow: 92%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
    Produktmerkmale
    This is a rabbit polyclonal antibody against Ncoa4. It was validated on Western Blot.
    Aufreinigung
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide corresponding to a region of Mouse
    Top Product
    Discover our top product NCOA4 Primärantikörper
  • Applikationshinweise
    Optimal working dilutions should be determined experimentally by the investigator.
    Kommentare

    Antigen size: 625 AA

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100057 (Western Blotting)
    'Independent Validation' Siegel
    by
    University of Michigan Medical School, Molecular & Integrative Physiology, Shah lab
    No.
    #100057
    Datum
    12.09.2016
    Antigen
    Ncoa4
    Chargennummer
    QC11327
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    HIF overexpression mice, WT mice
    Negativkontrolle
    NCOA4-/- mice
    Bewertung
    Passed, based on the fact that the antibody picked up the positive control (unpublished data suggests that NCOA4 is highly expressed in an intestinal HIF overexpression model).
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2780237
    Sekundärantikörper
    Anti-rabbit HRP-linked secondary antibody (Cell Signaling, 7074S, lot 26)
    Full Protocol
    • Wild type (WT) or NCOA4 -/- mice were fed normal or iron-deficient diet for 7 days, followed by euthanization and scraping of duodenal epithelium (DE).
    • DE cells were lysed in RIPA buffer and whole cell lysate (WCL) prepared.
    • 50µg total protein from each sample were used for Western blot analysis.
    • Samples were denatured in 5x gel-loading buffer containing beta-marcaptoethanol and separated on a 10% SDS-PAGE gel (Laemmli 1970).
    • Transfer onto Nitrocellulose membrane (Biorad, 1620212, lot 10484060) was done on Trans-Blot SD apparatus (Bio-Rad) following the manufacturer’s protocol.
    • Membranes were blocked with TBS-tween (TBST) solution containing 5% milk for 1h.
    • Incubation with primary antibody overnight at 4°C:
      • NCOA4 antibody ABIN2780237 (lot QC11327) diluted 1:1000 in TBST containing 0.1% BSA.
      • FTH1 antibody (Cell signaling, 3998S; lot 1) diluted in TBST containing 0.1% BSA.
      • GAPDH antibody (Santa Cruz, SC-25778, lot D30115) diluted in TBST containing 0.1% BSA.
    • Washing with TBST for 15min at RT.
    • Incubation with anti-rabbit HRP-linked secondary antibody (Cell Signaling, 7074S, lot 26) diluted 1:2000 in TBST a shaker for 1h at RT.
    • Three rinses, followed by four 15min washes, twice TBST and twice with TBS.
    • Chemiluminescence detection was done using ECL Select Western blotting detection reagent (Amersham, RPN2235, lot 9723881).
    • Blot was developed on a Biorad imaging system.
    Anmerkungen
    • The antibody did not consistently pick up the WT mice on normal and low iron diet.
    • Also, there is still a faint band in the NCOA4 -/- lane. We are confident that it is a true KO mouse, as there is high ferritin expression even after 7 days of low iron diet (compared to ferritin expression in WT low iron mice as well as that of the HIF over expression mouse, which are known to have high intestinal ftn content).
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    NCOA4 (Nuclear Receptor Coactivator 4 (NCOA4))
    Andere Bezeichnung
    Ncoa4 (NCOA4 Produkte)
    Synonyme
    zgc:55307 antikoerper, zgc:77270 antikoerper, NCOA4 antikoerper, MGC138109 antikoerper, rfg antikoerper, ele1 antikoerper, ptc3 antikoerper, ara70 antikoerper, MGC84996 antikoerper, DKFZp469A035 antikoerper, DKFZp459B2429 antikoerper, DKFZp469A1925 antikoerper, ncoa4 antikoerper, ARA70 antikoerper, ELE1 antikoerper, PTC3 antikoerper, RFG antikoerper, 1110034E15Rik antikoerper, 4432406M01Rik antikoerper, AI227008 antikoerper, Rfg antikoerper, nuclear receptor coactivator 4 antikoerper, nuclear receptor coactivator 4 S homeolog antikoerper, Ncoa4 antikoerper, ncoa4 antikoerper, NCOA4 antikoerper, ncoa4.S antikoerper
    Hintergrund
    The function of Ncoa4 remains unknown.
    Alias Symbols: 1110034E15Rik, 4432406M01Rik, AI227008, ARA70, MGC103382, Rfg
    Protein Interaction Partner: Ndn,
    Protein Size: 625
    Molekulargewicht
    70 kDa
    Gen-ID
    27057
    NCBI Accession
    NM_001033988, NP_001029160
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway
Sie sind hier:
Kundenservice