anti-Maus KI-67 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended KI-67 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Epitop
C-Term
92
54
49
18
18
15
15
14
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
457
112
71
17
16
9
3
2
1
Wirt
Kaninchen
260
158
32
9
5
3
Klonalität
Monoklonal
Konjugat
Dieser KI-67 Antikörper ist unkonjugiert
17
13
11
10
10
8
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
187
184
157
120
118
104
85
72
16
13
13
11
6
3
3
3
1
1
1
1
Hersteller
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Produktnummer ABIN269440
381,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.47111 ABIN677858 IF (cc) IF (p) IHC (p) Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal 16
11.47111 ABIN409932 ICC FACS IHC (fro) IF IHC (p) IHC WB Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal 31
11.47111 ABIN409934 FACS ICC IF IHC IHC (fro) IHC (p) WB Rabbit AA 1800-1850, Internal Region Anmelden zum Anzeigen Polyclonal 12
11.47111 ABIN269401 ICC IF IHC IHC (fro) IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 7
11.47111 ABIN4328638 FACS IHC IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 4
1 ABIN1687925 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6 379
1 ABIN1687135 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6 406
1 ABIN372054 IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen SP6
1 ABIN152984 ICC IHC (fro) IF IHC (p) IHC WB Rabbit IgG Internal Region, AA 1550-1700 Anmelden zum Anzeigen Polyclonal 58
1 ABIN409942 IHC (p) IHC Rabbit AA 2100-2200, Internal Region Anmelden zum Anzeigen Polyclonal 1
1 ABIN409940 ICC IF IHC IHC (fro) IHC (p) Rabbit AA 1200-1300, Internal Region Anmelden zum Anzeigen Polyclonal 8
1 ABIN4328651 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit IgG AA 1550-1700, Internal Region Anmelden zum Anzeigen Polyclonal 1
1 ABIN677867 IHC (p) HRP Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal
1 ABIN677860 IHC (p) Biotin Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal
1 ABIN5631305 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 594 Rabbit IgG AA 1550-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328632 ICC IF IHC IHC (fro) IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6
1 ABIN4328667 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328669 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328671 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328672 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
Epitop C-Term
(92), (54), (49), (18), (18), (15), (15), (14), (8), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(457), (112), (71), (17), (16), (9), (3), (2), (1)
Wirt Kaninchen
(260), (158), (32), (9), (5), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser KI-67 Antikörper ist unkonjugiert
(17), (13), (11), (10), (10), (8), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(187), (184), (157), (120), (118), (104), (85), (72), (16), (13), (13), (11), (6), (3), (3), (3), (1), (1), (1), (1)
Pubmed 20 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-KI-67 Antikörper

Target Details KI-67 Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN269440) Bilder
Spezifität Ki67 (SP6)
Reinigung Tissue culture supernatant
Immunogen A synthetic peptide from C-terminus of human Ki-67.
Klon SP6
Isotyp IgG

Target Details KI-67

Produktdetails anti-KI-67 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN269440) Bilder zurück nach oben
Antigen
Andere Bezeichnung Ki-67/MKI67 (MKI67 Antibody Abstract)
Hintergrund Gene Symbol: MKI67
Gen-ID 4288
Pathways Glycosaminoglycan Metabolic Process

Anwendungsinformationen

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN269440) Bilder zurück nach oben
Applikationshinweise Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen 1:10-1:500Suggested incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 20235278) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23300752)
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry-Paraffin Protocol Specific for NB600-1252: Ki67 Antibody (SP6)Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen ProductDetails: References for anti-KI-67 antibody (ABIN269440) Bilder zurück nach oben
Format Liquid
Buffer PBS and BSA
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.

ProductDetails: References for anti-KI-67 antibody (ABIN269440)

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Falkenberg, Höfig, Rosemann, Szumielewski, Richter, Schorpp, Hadian, Aubele, Atkinson, Anastasov: "Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer." in: Cancer medicine, Vol. 5, Issue 4, pp. 703-10, 2016 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Rath, Deb, Sengupta, Kari, Kar, Parbin, Pradhan, Patra: "Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin." in: Tumour biology, Vol. 37, Issue 9, pp. 12535-12546, 2016 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry

Kalliokoski, Piqueras, Frías, Sulic, Määttä, Kähkönen, Viiri, Huhtala, Pasternack, Laurila, Sblattero, Korponay-Szabó, Mäki, Caja, Kaukinen, Lindfors: "Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice." in: Amino acids, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Sumis, Cook, Andrade, Hu, Kidney, Zhang, Kim, Carney, Nguyen, Yu, Bouker, Cruz, Clarke, Hilakivi-Clarke: "Social isolation induces autophagy in the mouse mammary gland: link to increased mammary cancer risk." in: Endocrine-related cancer, Vol. 23, Issue 10, pp. 839-56, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Du, Bondarenko, Wang, Rouabhia, Zhang: "Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression." in: Journal of cellular physiology, Vol. 231, Issue 6, pp. 1301-12, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Migita, Yoshitake, Tange, Choijookhuu, Hishikawa: "Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats." in: Nephro-urology monthly, Vol. 8, Issue 1, pp. e34421, 2016 Von den Autoren verwendete Methode: Western Blotting (WB)

Ueha, Ueha, Sakamoto, Kanaya, Suzukawa, Nishijima, Kikuta, Kondo, Matsushima, Yamasoba: "Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice." in: Neurotoxicity research, Vol. 30, Issue 2, pp. 213-24, 2016 Von den Autoren verwendete Methode: Western Blotting (WB)

Ueha, Ueha, Kondo, Sakamoto, Kikuta, Kanaya, Nishijima, Matsushima, Yamasoba: "Damage to Olfactory Progenitor Cells Is Involved in Cigarette Smoke-Induced Olfactory Dysfunction in Mice." in: The American journal of pathology, Vol. 186, Issue 3, pp. 579-86, 2016

Penna, Gigoni, Costa, Vella, Russo, Poggi, Villa, Brizzolara, Canale, Mescola, Daga, Russo, Nizzari, Florio, Menichini, Pagano: "The inhibition of 45A ncRNA expression reduces tumor formation, affecting tumor nodules compactness and metastatic potential in neuroblastoma cells." in: Oncotarget, Vol. 8, Issue 5, pp. 8189-8205, 2016 Von den Autoren verwendete Methode: Immunohistochemistry (IHC) (Probematerial (Species): Mouse (Murine)).

Orticello, Fiore, Totta, Desideri, Barisic, Passeri, Lenzi, Rosa, Orlandi, Maiato, Del Bufalo, Degrassi: "N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics." in: Oncogene, Vol. 34, Issue 25, pp. 3325-35, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Papazoglou, Jean, Gertler, Taouis, Vacher: "Hippocampal GSK3β as a Molecular Link Between Obesity and Depression." in: Molecular neurobiology, Vol. 52, Issue 1, pp. 363-74, 2015

Kalliokoski, Caja, Frias, Laurila, Koskinen, Niemelä, Mäki, Kaukinen, Korponay-Szabó, Lindfors: "Injection of celiac disease patient sera or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 1, pp. 51-62, 2015

Schevzov, Kee, Wang, Sequeira, Hook, Coombes, Lucas, Stehn, Musgrove, Cretu, Assoian, Fath, Hanoch, Seger, Pleines, Kile, Hardeman, Gunning: "Regulation of cell proliferation by ERK and signal-dependent nuclear translocation of ERK is dependent on Tm5NM1-containing actin filaments." in: Molecular biology of the cell, Vol. 26, Issue 13, pp. 2475-90, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Cage, Chanthery, Chesler, Grimmer, Knight, Shokat, Weiss, Gustafson: "Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer." in: Frontiers in oncology, Vol. 5, pp. 111, 2015 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Kim, Wong, Amarnani, Bigger-Allen, Hu, Marko, Eliott, Shah, McGuone, Stemmer-Rachamimov, Gai, DAmore, Arboleda-Velasquez: "Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy." in: Molecular vision, Vol. 21, pp. 673-87, 2015 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Vesci, Carollo, Roscilli, Aurisicchio, Ferrara, Spagnoli, De Santis: "Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance." in: Oncology reports, Vol. 34, Issue 3, pp. 1146-52, 2015 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Vesci, Carollo, Roscilli, Aurisicchio, Ferrara, Spagnoli, De Santis: "[Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance." in: Oncology reports, Vol. 35, Issue 1, pp. 602, 2015

Tukler Henriksson, Coursey, Corry, De Paiva, Pflugfelder: "IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells." in: Investigative ophthalmology & visual science, Vol. 56, Issue 8, pp. 4186-97, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Hua, Schindler, McQuail, Forbes, Riddle: "Regionally distinct responses of microglia and glial progenitor cells to whole brain irradiation in adult and aging rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e52728, 2013 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Frozen Sections)

Vulpio, Bossola, Di Stasio, Tazza, Silvestri, Fadda: "Histology and immunohistochemistry of the parathyroid glands in renal secondary hyperparathyroidism refractory to vitamin D or cinacalcet therapy." in: European journal of endocrinology, Vol. 168, Issue 6, pp. 811-9, 2013 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Bilder

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN269440) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (C-Term) antibody (ABIN269440) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [ABIN269440] - Formalin fix...