anti-Maus KI-67 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended KI-67 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • marker of proliferation Ki-67
  • marker of proliferation Ki-67 L homeolog
  • marker of proliferation Ki-67 S homeolog
  • antigen identified by monoclonal antibody Ki 67
  • antigen identified by monoclonal antibody Ki-67
  • nucleolar protein interacting with the FHA domain of MKI67
  • MKI67
  • mki67
  • mki67.L
  • mki67.S
  • Mki67
  • NIFK
Epitop
AA 1600-1700, Internal Region
92
54
50
18
17
15
15
14
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
458
112
71
19
16
11
4
4
2
1
Wirt
Kaninchen
261
158
32
9
5
3
Klonalität
Polyklonal
Konjugat
Dieser KI-67 Antikörper ist unkonjugiert
17
13
11
10
10
8
7
7
6
5
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
188
183
157
122
118
104
84
72
16
13
13
11
6
3
3
3
1
1
1
1
Hersteller
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Produktnummer ABIN409932
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.796137 ABIN409934 FACS ICC IF IHC IHC (fro) IHC (p) WB Rabbit AA 1800-1850, Internal Region Anmelden zum Anzeigen Polyclonal 12
13.796137 ABIN269440 ICC IHC (fro) IF IHC (p) IHC Rabbit IgG C-Term Anmelden zum Anzeigen SP6 28
13.796137 ABIN269401 ICC IF IHC IHC (fro) IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 7
13.796137 ABIN677858 IF (cc) IF (p) IHC (p) Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal 16
13.796137 ABIN4328638 FACS IHC IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 4
1 ABIN1687925 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6 379
1 ABIN152984 ICC IHC (fro) IF IHC (p) IHC WB Rabbit IgG Internal Region, AA 1550-1700 Anmelden zum Anzeigen Polyclonal 58
1 ABIN409940 ICC IF IHC IHC (fro) IHC (p) Rabbit AA 1200-1300, Internal Region Anmelden zum Anzeigen Polyclonal 8
1 ABIN409942 IHC (p) IHC Rabbit AA 2100-2200, Internal Region Anmelden zum Anzeigen Polyclonal 1
1 ABIN1687135 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6 406
1 ABIN372054 IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen SP6
1 ABIN4328651 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit IgG AA 1550-1700, Internal Region Anmelden zum Anzeigen Polyclonal 1
1 ABIN677867 IHC (p) HRP Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal
1 ABIN677860 IHC (p) Biotin Rabbit IgG AA 1250-1300 Anmelden zum Anzeigen Polyclonal
1 ABIN4328630 ICC IF IHC IHC (fro) IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4328631 ICC IF IHC IHC (fro) IHC (p) WB Rabbit IgG AA 1550-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN5631305 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 594 Rabbit IgG AA 1550-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328632 ICC IF IHC IHC (fro) IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen SP6
1 ABIN4328669 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4328674 FACS ICC IF IHC IHC (fro) IHC (p) WB PerCP Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
Epitop AA 1600-1700, Internal Region
(92), (54), (50), (18), (17), (15), (15), (14), (8), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(458), (112), (71), (19), (16), (11), (4), (4), (2), (1)
Wirt Kaninchen
(261), (158), (32), (9), (5), (3)
Klonalität
Polyklonal
Konjugat Dieser KI-67 Antikörper ist unkonjugiert
(17), (13), (11), (10), (10), (8), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(188), (183), (157), (122), (118), (104), (84), (72), (16), (13), (13), (11), (6), (3), (3), (3), (1), (1), (1), (1)
Pubmed 20 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-KI-67 Antikörper

Target Details KI-67 Anwendungsinformationen Handhabung Referenzen für anti-KI-67 Antikörper (ABIN409932) Bilder
Reinigung Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the mouse Ki67 protein (within residues 1600-1700). [Swiss-Prot# Q61769]

Target Details KI-67

Produktdetails anti-KI-67 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-KI-67 Antikörper (ABIN409932) Bilder zurück nach oben
Antigen
Andere Bezeichnung Ki-67/MKI67 (MKI67 Antibody Abstract)
Hintergrund Gene Symbol: MKI67
Molekulargewicht Theoretical MW: 324 kDa
Gen-ID 4288
UniProt Q61769
Pathways Glycosaminoglycan Metabolic Process

Anwendungsinformationen

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Handhabung Referenzen für anti-KI-67 Antikörper (ABIN409932) Bilder zurück nach oben
Applikationshinweise Western Blot 1:100-1:2000, Flow Cytometry 1:100, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-FrozenThis Ki67 antibody is useful for Flow Cytometry, Western blot (PMID 22384261), Immunohistochemistry and Immunofluorescence/immunocytochemistry. Immunohistochemistry-Frozen was reported in scientific literature. This antibody was also shown to be useful in Flow Cytometry analysis by a customer. Please see the product review for further details. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry Protocol specific for Ki67 Antibody Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. Staining:1) Wash sections in dH2O three times for 5 minutes each.
2) Wash section in wash buffer (1X PBS/0.1 % Tween-20 (1X PBST)) for 5 minutes. 3) Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature. 4) Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C. 5) Remove antibody solution and wash sections in wash buffer three times for 5 minutes each. 6) Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature. 7) Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each. 8) Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature. 9) Wash sections three times in wash buffer for 5 minutes each. 10) Add 100-400 µL DAB substrate to each section and monitor staining closely. 11) As soon as the sections develop, immerse slides in dH2O. 12) Counterstain sections in hematoxylin. 13) Wash sections in dH2O two times for 5 minutes each. 14) Dehydrate sections. 15) Mount coverslips.Immunocytochemistry/Immunofluorescence protocol for Ki67 antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Referenzen für anti-KI-67 Antikörper (ABIN409932) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-KI-67 Antikörper (ABIN409932)

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Furukawa, Nagaike, Ozaki: "Databases for technical aspects of immunohistochemistry." in: Journal of toxicologic pathology, Vol. 30, Issue 1, pp. 79-107, 2017

Kai, Tsukamoto, Hijiya, Tokunaga, Nakada, Uchida, Daa, Iha, Takahashi, Nomura, Sato, Mimata, Ikawa, Seto, Matsuura, Moriyama: "Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway." in: The Journal of pathology, Vol. 239, Issue 1, pp. 97-108, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Yancu, Blouin, Birman, Florianova, Aleynikova, Zakikhani, VanderMeulen, Seidman, Pollak: "Insulin-like growth factor-3 knockout mice are resistant to experimental dextran sulfate sodium colitis." in: Journal of gastroenterology and hepatology, 2016 Weitere Details: Immunohistochemistry

Liang, Luo, Wei, Ma, Yang, Shao, Liu, Liu, Ren, Liu, He, Wei: "A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy." in: Oncotarget, 2016 Weitere Details: Immunohistochemistry

Chen, Chyau, Lee, Tseng, Chou: "Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells." in: Scientific reports, Vol. 6, pp. 20417, 2016 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Kalliokoski, Piqueras, Frías, Sulic, Määttä, Kähkönen, Viiri, Huhtala, Pasternack, Laurila, Sblattero, Korponay-Szabó, Mäki, Caja, Kaukinen, Lindfors: "Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice." in: Amino acids, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Beristain, Molyneux, Joshi, Pomroy, Di Grappa, Chang, Kirschner, Privé, Pujana, Khokha: "PKA signaling drives mammary tumorigenesis through Src." in: Oncogene, Vol. 34, Issue 9, pp. 1160-73, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Mathes, Rice, Affandi, DiMarzio, Rifkin, Stifano, Christmann, Lafyatis: "CpGB DNA activates dermal macrophages and specifically recruits inflammatory monocytes into the skin." in: Experimental dermatology, Vol. 24, Issue 2, pp. 133-9, 2015

Rachidi, Sun, Wu, Jones, Drake, Ogretmen, Cowart, Clarke, Hannun, Chiosis, Liu, Li: "Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis." in: Journal of hepatology, Vol. 62, Issue 4, pp. 879-88, 2015

Zhou, Rychahou, Wang, Weiss, Evers: "TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium." in: Cell death & disease, Vol. 6, pp. e1631, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Lindley, Curtis, Sanchez-Mejias, Rieger, Robbins, Briegel: "The WNT-controlled transcriptional regulator LBH is required for mammary stem cell expansion and maintenance of the basal lineage." in: Development (Cambridge, England), Vol. 142, Issue 5, pp. 893-904, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Aggarwal, Höbaus, Tennakoon, Prinz-Wohlgenannt, Graça, Price, Heffeter, Berger, Baumgartner-Parzer, Kállay: "Active vitamin D potentiates the anti-neoplastic effects of calcium in the colon: A cross talk through the calcium-sensing receptor." in: The Journal of steroid biochemistry and molecular biology, Vol. 155, Issue Pt B, pp. 231-8, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Nowak, Cho, Herzka, Watrud, DeMarco, Wang, Senturk, Fellmann, Ding, Beinortas, Kleinman, Chen, Sordella, Wilkinson, Castillo-Martin, Cordon-Cardo, Robinson, Trotman: "MYC Drives Pten/Trp53-Deficient Proliferation and Metastasis due to IL6 Secretion and AKT Suppression via PHLPP2." in: Cancer discovery, Vol. 5, Issue 6, pp. 636-51, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Velarde, Demaria, Melov, Campisi: "Pleiotropic age-dependent effects of mitochondrial dysfunction on epidermal stem cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 33, pp. 10407-12, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Franz, Greschik, Willmann, Ozretić, Jilg, Wardelmann, Jung, Buettner, Schüle: "The histone code reader SPIN1 controls RET signaling in liposarcoma." in: Oncotarget, Vol. 6, Issue 7, pp. 4773-89, 2015 Weitere Details: Immunocytochemistry,Immunofluorescence

Jones, Reens, Brocklehurst, Betts, Bickerton, Bigley, Jenkins, Whalley, Morgan, Smith: "Islets of Langerhans from prohormone convertase-2 knockout mice show α-cell hyperplasia and tumorigenesis with elevated α-cell neogenesis." in: International journal of experimental pathology, Vol. 95, Issue 1, pp. 29-48, 2014 (Probematerial (Species): Mouse (Murine)).

Kaenel, Hahnewald, Wotzkow, Strange, Andres: "Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization." in: Development, growth & differentiation, Vol. 56, Issue 4, pp. 255-75, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Fong, Martinez, Yang, Mikos, Navone, Harrington, Farach-Carson: "Hydrogel-based 3D model of patient-derived prostate xenograft tumors suitable for drug screening." in: Molecular pharmaceutics, Vol. 11, Issue 7, pp. 2040-50, 2014 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Laskov, Drudi, Beauchamp, Yasmeen, Ferenczy, Pollak, Gotlieb: "Anti-diabetic doses of metformin decrease proliferation markers in tumors of patients with endometrial cancer." in: Gynecologic oncology, Vol. 134, Issue 3, pp. 607-14, 2014 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Xiang, Gilkes, Chaturvedi, Luo, Hu, Takano, Liang, Semenza: "Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 2, pp. 151-64, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Bilder

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung Referenzen für anti-KI-67 Antikörper (ABIN409932) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [ABIN4099327] - Analysis of Ki-67...
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry: Ki-67/MKI67 Antibody [ABIN4099327] - Analysis of a mouse intest...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [ABIN4099327] - Staining of a cro...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [ABIN4099327] - analysis of Ki-67...
Flow Cytometry (FACS) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Flow Cytometry: Ki67 Antibody [ABIN4099327] - Staining of mouse bone marrow cells usi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [ABIN4099327] - analysis of Ki-67...
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry: Ki67 Antibody [ABIN4099327] - Detection of Ki67 in formalin-fix...
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunohistochemistry: Ki67 Antibody [ABIN4099327] - Immunohistochemical analysis of m...
Immunofluorescence (IF) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1600-1700), (Internal Region) antibody (ABIN409932) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN4099327] - Ki67 antibody w...