Human Granulocyte Chemotactic Protein 2 ELISA Kit

Recommended Granulocyte Chemotactic Protein 2 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kits
  • Cxcl6
  • AMCF-II
  • ENA-78
  • GCP-2
  • LIX
  • Scyb5
  • Scyb6
  • CKA-3
  • GCP2
  • SCYB6
  • CXCL5
  • chemokine (C-X-C motif) ligand 5
  • chemokine (C-X-C motif) ligand 6
  • chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
  • Cxcl5
  • CXCL6
Epitop
AA 40-114
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
18
9
9
3
2
2
2
2
1
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
31.2-2000 pg/mL
Untere Nachweisgrenze
31.2 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN1672728
$ 333.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.541325 ABIN1028752 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
10.541325 ABIN1115244 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
10.541325 ABIN2708186 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN367298 Sandwich ELISA Serum, Plasma, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN1873159 Sandwich ELISA Serum, Plasma, Biological Fluids 2.74-2.000 pg/mL 2.74 pg/mL Anmelden zum Anzeigen
1 ABIN5594913 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN511356 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 25-500 pg/mL 25 pg/mL Anmelden zum Anzeigen
1 ABIN5654802 Sandwich ELISA Biological Fluids, Plasma, Serum 31.25 pg/mL - 2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN5654803 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 15.6 pg/mL - 1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN5652433 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 39.1 pg/mL - 2500 pg/mL 39.1 pg/mL Anmelden zum Anzeigen
1 ABIN5525057 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN2042431 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN2684006 Sandwich ELISA Cell Lysate, Plasma, Serum Anmelden zum Anzeigen
1 ABIN2534090 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
1 ABIN455249 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN2944179 2.74-2000 pg/mL 2.74 pg/mL Anmelden zum Anzeigen
1 ABIN2945888 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN5072143 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kits
Epitop AA 40-114
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(18), (9), (9), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 31.2-2000 pg/mL
Untere Nachweisgrenze 31.2 pg/mL
Applikation ELISA
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL6/GCP2
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: V40-N114
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: V40-N114

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung CXCL6 (GCP2 ELISA Kit Abstract)
Hintergrund

Protein Function: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram- negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7). .

Background: Chemokine(C-X-C motif) ligand 6(CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2(GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

Synonyms: C-X-C motif chemokine 6,Chemokine alpha 3,CKA-3,Granulocyte chemotactic protein 2,GCP-2,Small-inducible cytokine B6,Small-inducible cytokine B6, N-processed variant 1,Small-inducible cytokine B6, N-processed variant 2,Small-inducible cytokine B6, N-processed variant 3,CXCL6,GCP2, SCYB6,

Full Gene Name: C-X-C motif chemokine 6

Cellular Localisation: Secreted.
Gen-ID 6372
UniProt P80162
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity

Anwendungsinformationen

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Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plattentyp Pre-coated
Protokoll human CXCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL6 has been precoated onto 96-well plates. Standards(E.coli, V40-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL6 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL6 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 223, Standard deviation: 13.6, CV(%): 6.1
  • Sample 2: n=16, Mean(pg/ml): 519, Standard deviation: 33.2, CV(%): 6.4
  • Sample 3: n=16, Mean(pg/ml): 1089, Standard deviation: 64.3, CV(%): 5.9,
  • Sample 1: n=24, Mean(pg/ml): 245, Standard deviation: 18.1, CV(%): 7.4
  • Sample 2: n=24, Mean(pg/ml): 542, Standard deviation: 41.2, CV(%): 7.6
  • Sample 3: n=24, Mean(pg/ml): 1054, Standard deviation: 71.7, CV(%): 6.8
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

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Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

ODonovan, Galvin, Morgan: "Physical mapping of the CXC chemokine locus on human chromosome 4." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 39-42, 1999

Modi, Chen: "Localization of the human CXC chemokine subfamily on the long arm of chromosome 4 using radiation hybrids." in: Genomics, Vol. 47, Issue 1, pp. 136-9, 1998

Wuyts, Van Osselaer, Haelens, Samson, Herdewijn, Ben-Baruch, Oppenheim, Proost, Van Damme: "Characterization of synthetic human granulocyte chemotactic protein 2: usage of chemokine receptors CXCR1 and CXCR2 and in vivo inflammatory properties." in: Biochemistry, Vol. 36, Issue 9, pp. 2716-23, 1997

Bilder

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Bilder des Herstellers
ELISA image for Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit (ABIN1672728) Human CXCL6/GCP2 PicoKine ELISA Kit standard curve