anti-Human TJP1 Antikörper für Immunofluorescence

Recommended TJP1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Epitop
AA 1048-1247
16
10
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
94
35
33
20
11
5
4
4
3
2
1
1
1
1
Wirt
Maus
77
16
3
2
2
Klonalität
Monoklonal
Konjugat
Dieser TJP1 Antikörper ist unkonjugiert
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
76
26
23
23
23
14
13
11
10
9
8
3
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN968282
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.14905 ABIN968283 IF WB Mouse IgG1 AA 1048-1247 Anmelden zum Anzeigen 1-ZO 4
12.14905 ABIN4359735 ICC IF IHC IHC (p) SimWes Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
12.14905 ABIN4359733 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 2
1 ABIN1109613 IHC (fro) FACS IF IHC (p) WB Rabbit Ig AA 886-995 Anmelden zum Anzeigen Polyclonal 5
1 ABIN1109612 IHC (fro) IF IHC (p) WB Guinea Pig Ig Anmelden zum Anzeigen Polyclonal 3
1 ABIN602574 IHC (fro) IF WB Rabbit AA 866-995 Anmelden zum Anzeigen Polyclonal
1 ABIN602573 IHC (fro) IF WB Rabbit AA 432-1150 Anmelden zum Anzeigen Polyclonal
1 ABIN2964417 IF IHC (p) WB Goat IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN199816 IHC (fro) IF WB Rabbit IgG Middle Region Anmelden zum Anzeigen Polyclonal
1 ABIN1875102 FACS IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4359734 ICC IF IHC IHC (p) SimWes Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2744882 ELISA IF WB FITC Mouse IgG1 AA 334-634 Anmelden zum Anzeigen ZO1-1A12
1 ABIN2629246 IF IHC (p) WB Goat IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4359736 ICC IF WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal
1 ABIN2887986 FACS IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4359732 ICC IF IHC IHC (fro) IHC (p) WB Guinea Pig Anmelden zum Anzeigen Polyclonal
1 ABIN4359731 ICC IF IHC IHC (fro) IHC (p) WB Guinea Pig Anmelden zum Anzeigen Polyclonal
1 ABIN2192206 IF Rabbit Anmelden zum Anzeigen Polyclonal 1
1 ABIN2394522 FACS IF WB Rabbit IgG AA 432-1150 Anmelden zum Anzeigen Polyclonal
1 ABIN5589544 IF IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal

General

Antigen Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
Epitop AA 1048-1247
(16), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(94), (35), (33), (20), (11), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Wirt Maus
(77), (16), (3), (2), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TJP1 Antikörper ist unkonjugiert
(3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (IF), Western Blotting (WB)
(76), (26), (23), (23), (23), (14), (13), (11), (10), (9), (8), (3), (2), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TJP1 Antikörper

Target Details TJP1 Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN968282) Bilder
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human ZO-1 aa. 1048-1247
Klon 1-ZO
Isotyp IgG1

Target Details TJP1

Produktdetails anti-TJP1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN968282) Bilder zurück nach oben
Antigen
Andere Bezeichnung ZO-1 (TJP1 Antibody Abstract)
Hintergrund Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intracellular barriers between epithelial and endothelial cells. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1, and ZO-2. Analysis of ZO-1 and -2 resulted in their inclusion in the MAGUK protein family. This family also includes the discs large tumor suppressor protein (Dlg-A) of Drosophila, p55, an erythrocyte membrane protein, and PSD-95/SAP90, a synaptic membrane protein. All family members contain a region homologous to guanylate kinase (GuK), a src homology (SH3) domain, and multiple PDZ domains. Through these elements, MAGUK proteins function in signal transduction and, possibly, tumor suppression. ZO-1 is a peripheral membrane phosphoprotein that binds to other tight junction proteins such as occludin and AF-6. Via its SH3 domain, ZO-1 interacts with a serine protein kinase that phosphorylates a region immediately C-terminal of the SH3 domain. Taken together, these data indicate that ZO-1 is a critical element in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.
Molekulargewicht 220 kDa
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization

Anwendungsinformationen

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Handhabung Referenzen für anti-TJP1 Antikörper (ABIN968282) Bilder zurück nach oben
Kommentare

Related Products: ABIN968535, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Referenzen für anti-TJP1 Antikörper (ABIN968282) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-TJP1 Antikörper (ABIN968282)

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hirase, Kawashima, Wong, Ueyama, Rikitake, Tsukita, Yokoyama, Staddon: "Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10423-31, 2001

Nix, Chishti, Anderson, Walther: "hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41192-200, 2001

Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, 2000

Willott, Balda, Fanning, Jameson, Van Itallie, Anderson: "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7834-8, 1993

Bilder

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN968282) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Western blot analysis of ZO-1 on a HeLa cell lysate. Lane 1: 1:250, lane 2: 1:500, la...
Immunofluorescence (IF) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Immunofluorescent staining of EAHY human endothelial cells.