anti-Maus FASL Antikörper für Immunofluorescence

Recommended FASL Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Reaktivität
Human, Maus
421
173
89
36
7
5
5
3
3
2
1
1
Wirt
Ratte
259
176
49
32
28
8
1
Klonalität
Monoklonal
Konjugat
Dieser FASL Antikörper ist unkonjugiert
45
31
27
12
12
9
9
9
9
8
8
8
8
8
7
6
6
4
3
2
2
2
2
2
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
313
257
125
97
80
75
51
46
28
28
13
12
10
10
7
5
3
2
2
1
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN187289
$ 587.40
Zzgl. Versandkosten $45.00
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General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
Reaktivität Human, Maus
(421), (173), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Wirt Ratte
(259), (176), (49), (32), (28), (8), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser FASL Antikörper ist unkonjugiert
(45), (31), (27), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(313), (257), (125), (97), (80), (75), (51), (46), (28), (28), (13), (12), (10), (10), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FASL Antikörper

Target Details FASL Anwendungsinformationen Handhabung Bilder
Spezifität Species Reactivity: Mouse. Others not tested.
Reinheit > 95 % by SDS-PAGE.
Immunogen Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).
Klon H11
Isotyp IgG2a

Target Details FASL

Produktdetails anti-FASL Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung CD178 / Fas Ligand (FASL Antibody Abstract)
Hintergrund Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-FASL Antikörper Target Details FASL Handhabung Bilder zurück nach oben
Applikationshinweise Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C

Bilder

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody
Immunofluorescence (IF) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 2)
Western Blotting (WB) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 3)