Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202) Antikörper

Details zu Produkt Nr. ABIN968835, Anbieter: Anmelden zum Anzeigen
Antigen
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
Epitop
pThr202
128
127
35
34
30
19
18
15
15
15
15
15
12
9
9
9
9
8
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
421
320
304
57
23
15
9
8
8
7
7
6
5
5
3
3
2
2
2
1
1
1
1
Wirt
Maus
350
77
7
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
12
11
11
10
7
7
7
7
6
6
6
6
6
6
2
2
2
2
1
1
1
1
1
1
1
Applikation
BioImaging (BI), Intracellular Staining (ICS), Western Blotting (WB)
305
114
83
78
60
56
46
42
16
9
6
4
4
4
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Phosphorylated Rat ERK1 (T202/Y204) Peptide
Klon 20A
Isotyp IgG1
Kreuzreaktivität Human, Maus
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung ERK1/2 (MAPK1/3 Antibody Abstract)
Hintergrund The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers.
The 20A monoclonal antibody recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and pT184/pY186 of human ERK2. The orthologous phosphorylation sites in murine ERK1 and ERK2 are T203/Y205 and T183/Y185.
Synonyms: p44/42 MAPK, Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Molekulargewicht 44/42 kDa
Forschungsgebiet Signaling, Inflammation
Applikationshinweise Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
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Related Products: ABIN968534, ABIN968533, ABIN967700, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.
Bilder des Herstellers
 image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202) antibody (ABIN968835) A431 (ATCC CRL-1555) cells were either left untreated (lane 1) or treated (lane 2) wi...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202) antibody (ABIN968835) Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 we...
Produkt verwendet in: Meng, Casey: "Activation of Gz attenuates Rap1-mediated differentiation of PC12 cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43417-24, 2002 (PubMed).

Sivaraman, Wang, Nuovo, Malbon: "Hyperexpression of mitogen-activated protein kinase in human breast cancer." in: The Journal of clinical investigation, Vol. 99, Issue 7, pp. 1478-83, 1997 (PubMed).

Clark, Hynes: "Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14814-8, 1996 (PubMed).

Boulton, Cobb: "Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies." in: Cell regulation, Vol. 2, Issue 5, pp. 357-71, 1991 (PubMed).

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