Tumor Protein P53 (TP53) Antikörper

Antigen: Tumor Protein P53 (TP53)

Synonyme: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Klonalität: Monoklonal (BP53-12)

Wirt: Maus

Reaktivität: Human, Primaten

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN94448

Produktbeschreibung

Weitere Bezeichnung: p53

Immunogen: Bacterially expressed full-length wild-type p53

Format: Purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: BP53-12

Beschreibung: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. Itmediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulatedto activate transcription of specific genes, resulting in expression of p21waf1 and otherproteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. Thestructure of p53 comprises N-terminal transactivation domain, central DNA-binding domain,oligomerisation domain, and C-terminal regulatory domain. There are variousphosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53activation and stabilization.

Spezifität: The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDatumour suppressor found in increased amounts in a wide variety of transformed cells, it isfrequently mutated or inactivated in many types of cancer.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1-2 µg/ml, overnight in 4 o C Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel).

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Zellzyklus, Transkriptionsfaktoren, DNS/RNS, Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Confocal microscopy of human HeLa cells using anti-p53 (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic