WNT2 Antikörper (AA 221-320)
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- Target Alle WNT2 Antikörper anzeigen
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
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Bindungsspezifität
- AA 221-320
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser WNT2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Kreuzreaktivität
- Human, Maus
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human WNT2
- Isotyp
- IgG
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Histopathology and Tissue Shared Resource, Georgetown Lombardi Comprehensive Cancer Center
- No.
- #029629
- Datum
- 16.03.2014
- Antigen
- Chargennummer
- 131105
- Validierte Anwendung
- Immunohistochemistry
- Positivkontrolle
- Human glioma tissue
- Negativkontrolle
- Human brain
- Bewertung
- Signal was detected in positive control tissue and not in negative control tissue.
- Primärantikörper
- Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
- Catalog number: ABIN762896
- Lot number: 131105
- Sekundärantikörper
- Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
- Batch number: 10082183
- Full Protocol
- Immunohistochemistry was performed by hand.
- Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
- Sections were rinsed in de-ionized water for 5 min at RT.
- Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
- Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
- Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
- Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
- Sections were rinsed in TBST for 5 min 2X at RT.
- Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
- Sections were rinsed in TBST for 5 min 2X at RT.
- Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
- Sections were washed x 1 in Distilled Water.
- Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
- Sections were washed x 1 in Distilled Water.
- Sections were blued in Ammonium Hydroxide for 1 min.
- Sections were washed x 1 in Distilled Water.
- Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
- Anmerkungen
- The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
- The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
Validierung #029629 (Immunohistochemistry)ValidierungsbilderProtokoll -
- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).
: "
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Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).
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- Target
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
- Andere Bezeichnung
- WNT2 (WNT2 Produkte)
- Synonyme
- CG1916 antikoerper, D-wnt-2 antikoerper, DWnt-2 antikoerper, DWnt2 antikoerper, Dm DWnt2 antikoerper, Dm-2 antikoerper, Dmel\\CG1916 antikoerper, Dwnt-2 antikoerper, Wnt antikoerper, Wnt-2 antikoerper, dWnt2 antikoerper, wnt2 antikoerper, WNT2 antikoerper, irp antikoerper, Xwnt2 antikoerper, wnt-2 antikoerper, Xwnt-2 antikoerper, int1l1 antikoerper, 2610510E18Rik antikoerper, Int1l1 antikoerper, Irp antikoerper, Mirp antikoerper, Wnt2a antikoerper, INT1L1 antikoerper, IRP antikoerper, ZfWnt2 antikoerper, Wnt oncogene analog 2 antikoerper, Wnt family member 2 antikoerper, wingless-type MMTV integration site family member 2 antikoerper, wingless-type MMTV integration site family, member 2 antikoerper, Wnt2 antikoerper, wnt2 antikoerper, WNT2 antikoerper
- Hintergrund
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Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2
Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
- Gen-ID
- 7472
- UniProt
- P09544
- Pathways
- WNT Signalweg
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