Tissue factor Antikörper (AA 32-100)
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- Target Alle Tissue factor (F3) Antikörper anzeigen
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
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Bindungsspezifität
- AA 32-100
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Reaktivität
- Human, Maus, Ratte, Schwein
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Tissue factor Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Kreuzreaktivität
- Human, Maus, Schwein, Ratte
- Homologie
- Dog,Cow,Horse,Rabbit,Guinea Pig
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human CD142
- Isotyp
- IgG
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Reveal Biosciences
- No.
- #029589
- Datum
- 07.02.2014
- Antigen
- Chargennummer
- 980886W
- Validierte Anwendung
- Immunohistochemistry
- Positivkontrolle
- Mouse lung tissue
- Negativkontrolle
- Mouse brain tissue
- Bewertung
- Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
- Primärantikörper
- Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
- Catalog number: ABIN708086
- Lot number: 120224
- Sekundärantikörper
- Antibody: Bond Polymer Refine Detection Kit
- Lot number: 24144
- Full Protocol
- Immunohistochemistry was performed on a Leica Bond automated immunostainer.
- Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
- Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
- Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
- Sections were washed x 3 in Leica Bond Wash Buffer.
- Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
- Sections were washed x 3 in Leica Bond Wash Buffer.
- Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
- Sections were washed x 4 in Leica Bond Wash Buffer.
- Sections were washed x 1 in Distilled Water.
- Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
- Sections were washed x 4 in Leica Bond Wash Buffer.
- Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
- Sections were washed x 3 in Distilled Water.
- Sections were counterstained with hematoxylin (Leica) for 2 minutes.
- Sections were washed x 1 in Distilled Water.
- Sections were washed x 1 in Leica Bond Wash Buffer.
- Sections were washed x 1 in Distilled Water.
- Sections were dehydrated, mounted and photographed under a light microscope.
- Anmerkungen
- None notes.
Validierung #029589 (Immunohistochemistry)ValidierungsbilderProtokoll -
- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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-
Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).
: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).
: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).
: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).
: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).
: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).
: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).
: "
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Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).
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- Target
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
- Andere Bezeichnung
- CD142 (F3 Produkte)
- Synonyme
- CD142 antikoerper, TF antikoerper, TFA antikoerper, PRO1557 antikoerper, PRO2086 antikoerper, TFQTL1 antikoerper, f3 antikoerper, AA409063 antikoerper, Cf-3 antikoerper, Cf3 antikoerper, tf antikoerper, zgc:112151 antikoerper, coagulation factor III, tissue factor antikoerper, transferrin antikoerper, coagulation factor IIIa antikoerper, coagulation factor III antikoerper, tissue factor antikoerper, coagulation factor IIIb antikoerper, coagulation factor III (thromboplastin, tissue factor) S homeolog antikoerper, F3 antikoerper, TF antikoerper, f3a antikoerper, tf antikoerper, f3b antikoerper, f3.S antikoerper
- Hintergrund
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Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3
Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
- Gen-ID
- 2152
- UniProt
- P13726
- Pathways
- Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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