His Tag Antikörper

Details zu Produkt Nr. ABIN559683, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Maus
410
185
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
58
45
36
14
13
11
10
10
9
9
9
9
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
549
388
163
109
78
78
74
26
13
11
8
7
6
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Sequenz HHHHHH
Klon HIS-H8
Isotyp IgG2b
Spezifität Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivität WB (with ECL): 1-2ng of His-tagged fusion proteins
Reinigung Protein A affinity chromatography from mouse ascites fluid
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Applikationshinweise WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-His Tag antibody (ABIN559683) LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins i...
 image for anti-His Tag antibody (ABIN559683) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, pro...
 image for anti-His Tag antibody (ABIN559683) Standard ladder containing five different His-tagged proteins, untransfected control ...
Produkt verwendet in: Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri, Alnemri: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Allgemeine Veröffentlichungen Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

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