ING3 Antikörper (Isoform 1)

Produktnummer ABIN118056

Produktdetails anti-ING3 Antikörper

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Bindungsspezifität: AA 294-304, Isoform 1

Reaktivität: Human, Maus, Ratte, Hund, Huhn, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ING3 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Spezifität: This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. The sequence is not present in isoforms 2 and 3 (see below). However, 100 % homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known. Human p47 ING3 isoform 1, 418 aa, predicted MW 46.7 kDa: 1 mlyledylem ieqlpmdlrd rftemremdl qvqnamdqle qrvseffmna kknkpewree 61 qmasikkdyy kaledadekv qlanqiydlv drhlrkldqe lakfkmelea dnagiteile 121 rrsleldtps qpvnnhhahs htpvekrkyn ptshhtttdh ipekkfksea llstltsdas 181 kentlgcrnn nstassnnay nvnssqplgs ynigslssgt gagaitmaaa qavqataqmk 241 egrrtsslka syeafknndf qlgkefsmar etvgyssssa lmttltqnas ssaadsrsgr 301 ksknnnksss qqssssssss slsscsssst vvqeisqqtt vvpesdsnsq vdwtydpnep 361 rycicnqvsy gemvgcdnqd cpiewfhygc vglteapkgk wycpqctaam krrgsrhk

Aufreinigung: Immunoaffinity chromatography.

Immunogen: Synthetic peptide corresponding aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 40,000) againstthe immunizing peptide. Specific conditions for Western blotting (1: 200-1: 2,000) reactivityshould be optimized by the end user. Expect a band at approximately 47 kDa in sizecorresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate orextract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (UVabs. at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Details zu ING3

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Andere Bezeichnung: ING3 (ING3 Produkte)

Synonyme: ING3 antikoerper, Eaf4 antikoerper, ING2 antikoerper, MEAF4 antikoerper, p47ING3 antikoerper, 1300013A07Rik antikoerper, P47ING3 antikoerper, zgc:56327 antikoerper, inhibitor of growth family member 3 antikoerper, inhibitor of growth family, member 3 antikoerper, inhibitor of growth protein 3 antikoerper, inhibitor of growth family member 3 L homeolog antikoerper, ING3 antikoerper, Ing3 antikoerper, CpipJ_CPIJ009396 antikoerper, ing3 antikoerper, ing3.L antikoerper

Hintergrund: P47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.Synonyms: HSPC301, Inhibitor of growth protein 3, p47ING3

Gen-ID: 54556

NCBI Accession: NP_061944

UniProt: Q9NXR8