IL27 Antikörper (Interleukin-27 Subunit alpha) (HRP)

Details for Product anti-IL27 Antibody No. ABIN5551711, Anbieter: Anmelden zum Anzeigen
Antigen
  • IL-27
  • IL-27p28
  • Il30
  • p28
  • IL-27A
  • IL27A
  • IL27p28
  • IL30
  • interleukin 27
  • Il27
  • IL27
Reaktivität
Maus
13
6
Wirt
Kaninchen
8
6
1
Klonalität
Polyklonal
Konjugat
Dieser IL27 Antikörper ist konjugiert mit HRP
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
7
5
4
2
2
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Full length recombinant mouse IL27/p28 protein
Isotyp IgG
Spezifität This antibody reacts to Interleukin-27A.
Kreuzreaktivität (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.
Produktmerkmale Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Reinigung Ion exchange chromatography
Andere Bezeichnung Interleukin-27 / IL27A (IL27 Antibody Abstract)
Hintergrund The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.Synonyms: IL-27 subunit alpha, IL-30, IL27-A, IL30, Interleukin-30, p28
Gen-ID 246779
NCBI Accession NP_663611
UniProt Q8K3I6
Applikationshinweise ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 0.1 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative (Do NOT add Sodium Azide!)
Konservierungsmittel Gentamicin sulfate
Vorsichtsmaßnahmen This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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