eIF4EBP1 Antikörper (AA 101-118)

Produktnummer ABIN462434

Der Kaninchen Polyklonal anti-eIF4EBP1 Antikörper wird verwendet zum Nachweis von eIF4EBP1 in Proben von Human, Maus, Ratte, Kaninchen und Hamster. Er wurde validiert für WB, IHC, IHC (p) und EMSA.

Kurzübersicht für eIF4EBP1 Antikörper (AA 101-118) (ABIN462434)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reaktivität: Human, Maus, Ratte, Kaninchen, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser eIF4EBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Electrophoretic Mobility-Shift Assay (EMSA)

Produktdetails anti-eIF4EBP1 Antikörper

Bindungsspezifität: AA 101-118

Spezifität: Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Approved: GS, IHC, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: BBS,  pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Handhabung: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Details zu eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Andere Bezeichnung: EIF4EBP1 / 4EBP1

Hintergrund: Name/Gene ID: EIF4EBP1

Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1

Gen-ID: 1978

UniProt: Q13541

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling