PDCD4 Antikörper (pSer457)

Produktnummer ABIN400797

Produktdetails anti-PDCD4 Antikörper

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Bindungsspezifität: pSer457

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Xenopus (100 %).
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like

Aufreinigung: Protein A Chromatography

Klon: 9G6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4 (PDCD4 Produkte)

Synonyme: H731 antikoerper, D19Ucla1 antikoerper, Ma3 antikoerper, Tis antikoerper, Dug antikoerper, cb45 antikoerper, pdcd4 antikoerper, sb:cb45 antikoerper, wu:fc84b08 antikoerper, h731 antikoerper, MGC69337 antikoerper, PDCD4 antikoerper, wu:fa55g03 antikoerper, wu:fb08a11 antikoerper, wu:fe01a02 antikoerper, zgc:66311 antikoerper, programmed cell death 4 antikoerper, programmed cell death 4b antikoerper, programmed cell death 4 L homeolog antikoerper, programmed cell death 4 (neoplastic transformation inhibitor) antikoerper, programmed cell death 4a antikoerper, PDCD4 antikoerper, Pdcd4 antikoerper, pdcd4b antikoerper, pdcd4.L antikoerper, pdcd4 antikoerper, pdcd4a antikoerper

Hintergrund: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gen-ID: 27250

UniProt: Q53EL6