HA-Tag Antikörper

Details zu Produkt Nr. ABIN387711, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
444
33
20
2
1
1
1
1
Wirt
Maus
204
181
40
34
20
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
45
31
24
10
10
9
9
8
7
7
7
7
6
6
6
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
390
201
122
118
85
82
70
40
15
13
11
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke THE™
Immunogen A KLH coupled synthetic peptide (YPYDVPDYA)
Sequenz YPYDVPDYA
Klon 5E11D8
Isotyp IgG1
Spezifität THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
Produktmerkmale Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
Reinigung Protein A affinity column
Andere Bezeichnung HA Tag
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Bilder des Herstellers
 image for anti-HA-Tag antibody (ABIN387711) Lane 1-3: GST fusion protein in E. coli lysate (~ 31 kD) Lane 4: Negative E. coli lys...
Produkt verwendet in: Cheng, Yu, Liu, Deng, Ma, Wang: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, 2015 (PubMed).

Tanner, Liu, Oberste, Pallansch, Collett, Kirkegaard: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, 2014 (PubMed).

Pillay, Selland, Fleisch, Leighton, Cheng, Famulski, Ritzel, March, Wang, Allison, Waskiewicz: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, 2013 (PubMed).

Shulga, Anderson, Topham, Epand: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, 2012 (PubMed).

Borroto-Escuela, Romero-Fernandez, García-Negredo, Correia, Garriga, Fuxe, Ciruela: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, 2011 (PubMed).

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