HA-Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN387712, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
441
33
20
2
1
1
1
1
Wirt
Maus
202
181
40
33
20
3
Klonalität (Klon)
Monoklonal ()
Konjugat
HRP
44
31
24
10
10
9
9
8
7
7
7
7
6
6
6
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
389
200
121
117
86
81
71
40
15
13
11
8
7
5
4
4
4
3
2
2
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke THE™
Immunogen A synthetic peptide (YPYDVPDYA) coupled - KLH
Sequenz YPYDVPDYA
Klon 5E11D8
Isotyp IgG
Spezifität THETM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
Produktmerkmale The antibody is suitable for detecting the expression level of HA in fusion proteins. Do not add azide as it inhibits HRP activity.
Reinigung Protein A affinity column
Andere Bezeichnung HA Tag
Substanzklasse Tag
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-0.5 µg/mL
Western blot: 0.1-0.5 µg/mL
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
 image for anti-HA-Tag antibody (HRP) (ABIN387712) Lane 1-3: 2 µL, 0.4 µL, 0.08 µL N-terminal HA-GST fusion protein in E. coli cell lysa...
 image for anti-HA-Tag antibody (HRP) (ABIN387712) 293 cells transfected with N-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
 image for anti-HA-Tag antibody (HRP) (ABIN387712) 293 cells transfected with C-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
Produkt verwendet in: Haac, Anderson, Eggleston, Myles, Adelman: "The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3688-700, 2015 (PubMed).

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