His Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN387701, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Maus
410
185
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
HRP
57
45
36
17
14
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
549
388
164
109
78
78
74
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke THE™
Immunogen A synthetic peptide HHHHHH coupled - KLH
Sequenz HHHHHH
Klon 6G2A9
Isotyp IgG
Spezifität THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Produktmerkmale This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Reinigung Protein A affinity column
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
 image for anti-His Tag antibody (HRP) (ABIN387701) Lane 1: MarkerLane 2: 10 ng Multiple Tag Cell Lysate fusion protein (ABIN1536505) Lan...
Produkt verwendet in: Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, 2015 (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, 2015 (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, 2014 (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, 2014 (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, 2013 (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, 2012 (PubMed).

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