EIF4G1 Antikörper (pSer1232)

Produktnummer ABIN362450

Produktdetails anti-EIF4G1 Antikörper

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Bindungsspezifität: pSer1232

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4G1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: The antibody detects endogenous level of eIF4G only when phosphorylated at serine 1232.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer1232 (P-V-S (p) -P-L) derived from Human eIF4G. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Andere Bezeichnung: eIF4G (EIF4G1 Produkte)

Synonyme: EIF4GI antikoerper, CG10811 antikoerper, Dmel\\CG10811 antikoerper, EIF4G antikoerper, Eif4G antikoerper, deIF4G antikoerper, eIF-4G antikoerper, eIF4g antikoerper, p200 antikoerper, EIF4G1 antikoerper, wu:fb50a05 antikoerper, wu:fc60b06 antikoerper, eif4g antikoerper, CUCUMOVIRUS MULTIPLICATION 2 antikoerper, CUM2 antikoerper, EUKARYOTIC TRANSLATION INITIATION FACTOR 4G antikoerper, eukaryotic translation initiation factor 4G antikoerper, DDBDRAFT_0217646 antikoerper, DDBDRAFT_0217647 antikoerper, DDBDRAFT_0234257 antikoerper, DDB_0217646 antikoerper, DDB_0217647 antikoerper, DDB_0234257 antikoerper, NV18824 antikoerper, EIF-4G1 antikoerper, EIF4F antikoerper, P220 antikoerper, PARK18 antikoerper, p220 antikoerper, E030015G23Rik antikoerper, eIF4GI antikoerper, eukaryotic translation initiation factor 4 gamma 1 antikoerper, eukaryotic translation initiation factor 4G antikoerper, eukaryotic translation initiation factor 4 gamma, 1a antikoerper, eukaryotic translation initiation factor 4 gamma, 1 S homeolog antikoerper, eukaryotic initiation factor antikoerper, eukaryotic translation initiation factor 4 gamma antikoerper, eukaryotic translation initiation factor 4 gamma, 1 antikoerper, eukaryotic translation initiation factor 4, gamma 1 antikoerper, EIF4G1 antikoerper, eIF4G antikoerper, eif4g1a antikoerper, LOC704923 antikoerper, eif4g1.S antikoerper, EIF4G antikoerper, eIF4g antikoerper, Eif4g antikoerper, Eif4g1 antikoerper

Hintergrund: EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3, in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

Molekulargewicht: 220 kDa

NCBI Accession: NP_004944

UniProt: Q04637