eIF4EBP1 Antikörper (pThr45)

Produktnummer ABIN362265

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-eIF4EBP1 Antikörper (ABIN362265) detektiert spezifisch eIF4EBP1 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für eIF4EBP1 Antikörper (pThr45) (ABIN362265)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser eIF4EBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-eIF4EBP1 Antikörper

Bindungsspezifität: pThr45

Spezifität: The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr45 (S-T-T (p) -P-G) derived from Human 4E-BP1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-eIF4EBP1 Antikörper (ABIN362265)

Cao, Gong, Guo, Mietzsch, Kuang, Kwartler, Safi, Estrera, Gambello, Milewicz: "Thoracic aortic disease in tuberous sclerosis complex: molecular pathogenesis and potential therapies in Tsc2+/- mice." in: Human molecular genetics, Vol. 19, Issue 10, pp. 1908-20, (2010) (PubMed).

Details zu eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Andere Bezeichnung: 4E-BP1

Hintergrund: 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. Published Papers Jiumei Cao

Molekulargewicht: 18 kDa

NCBI Accession: NP_004086

UniProt: Q13541

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling