BIK Antikörper (BCL2-Interacting Killer (Apoptosis-Inducing)) (pThr33)

Details for Product anti-BIK Antibody No. ABIN358090, Anbieter: Anmelden zum Anzeigen
Antigen
  • BIK
  • Biklk
  • Blk
  • Nbk
  • BIP1
  • BP4
  • NBK
  • si:dkey-31f5.6
  • BCL2-interacting killer (apoptosis-inducing)
  • BCL2-interacting killer
  • BIK
  • Bik
  • bik
Epitop
pThr33
45
21
14
9
6
3
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
121
34
10
3
Wirt
Kaninchen
113
9
Klonalität
Polyklonal
Konjugat
Dieser BIK Antikörper ist unkonjugiert
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
103
62
53
30
14
14
9
9
4
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T33 of human Bik.
Isotyp Ig
Spezifität This antibody detects BIK pThr33.
Kreuzreaktivität (Details) Species reactivity (tested):Human.
Reinigung Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung BIK (BIK Antibody Abstract)
Hintergrund The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.Synonyms: Apoptosis inducer NBK, BIP1, BP4, Bcl-2-interacting killer, NBK
Molekulargewicht 18016 Da
Gen-ID 638, 9606
UniProt Q13323
Pathways Positive Regulation of Endopeptidase Activity
Applikationshinweise ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-BCL2-Interacting Killer (Apoptosis-Inducing) (BIK) (pThr33) antibody (ABIN358090) anti-BCL2-Interacting Killer (Apoptosis-Inducing) (BIK) (pThr33) antibody
 image for anti-BCL2-Interacting Killer (Apoptosis-Inducing) (BIK) (pThr33) antibody (ABIN358090) anti-BCL2-Interacting Killer (Apoptosis-Inducing) (BIK) (pThr33) antibody (Image 2)
Allgemeine Veröffentlichungen Nikrad, Johnson, Puthalalath, Coultas, Adams, Kraft: "The proteasome inhibitor bortezomib sensitizes cells to killing by death receptor ligand TRAIL via BH3-only proteins Bik and Bim." in: Molecular cancer therapeutics, Vol. 4, Issue 3, pp. 443-9, 2005 (PubMed).

Dong, Pirbhai, Xiao, Zhong, Wu, Zhong: "Degradation of the proapoptotic proteins Bik, Puma, and Bim with Bcl-2 domain 3 homology in Chlamydia trachomatis-infected cells." in: Infection and immunity, Vol. 73, Issue 3, pp. 1861-4, 2005 (PubMed).

Hur, Chesnes, Coser, Lee, Geck, Isselbacher, Shioda: "The Bik BH3-only protein is induced in estrogen-starved and antiestrogen-exposed breast cancer cells and provokes apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2351-6, 2004 (PubMed).

Gillissen, Essmann, Graupner, Stärck, Radetzki, Dörken, Schulze-Osthoff, Daniel: "Induction of cell death by the BH3-only Bcl-2 homolog Nbk/Bik is mediated by an entirely Bax-dependent mitochondrial pathway." in: The EMBO journal, Vol. 22, Issue 14, pp. 3580-90, 2003 (PubMed).

Arena, Martini, Luongo, Capelli, Larocca: "Mutations of the BIK gene in human peripheral B-cell lymphomas." in: Genes, chromosomes & cancer, Vol. 38, Issue 1, pp. 91-6, 2003 (PubMed).

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