E-cadherin Antikörper

Produktnummer ABIN335398

Produktdetails anti-E-cadherin Antikörper

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E-cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Human.

Aufreinigung: Purified

Immunogen: MB2 is a mouse monoclonal IgG2b antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with MCF-7/AZ cells expressing E-cadherin at their cell surface.

Klon: MB2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: MB2 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion. MB2 is useful for flow cytometry, immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-E-cadherin Antikörper (ABIN335398)

Rong, Boterberg, Maubach, Stove, Depypere, Van Slambrouck, Serreyn, De Keukeleire, Mareel, Bracke: "8-Prenylnaringenin, the phytoestrogen in hops and beer, upregulates the function of the E-cadherin/catenin complex in human mammary carcinoma cells." in: European journal of cell biology, Vol. 80, Issue 9, pp. 580-5, (2001) (PubMed).

Steelant, Goeman, Philippé, Oomen, Hilkens, Krzewinski-Recchi, Huet, Van der Eycken, Delannoy, Bruyneel, Mareel: "Alkyl-lysophospholipid 1-O-octadecyl-2-O-methyl- glycerophosphocholine induces invasion through episialin-mediated neutralization of E-cadherin in human mammary MCF-7 cells in vitro." in: International journal of cancer. Journal international du cancer, Vol. 92, Issue 4, pp. 527-36, (2001) (PubMed).

Bracke, Vyncke, Bruyneel, Vermeulen, De Bruyne, Van Larebeke, Vleminckx, Van Roy, Mareel: "Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro." in: British journal of cancer, Vol. 68, Issue 2, pp. 282-9, (1993) (PubMed).

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: E-Cadherin / Cadherin-1 (CDH1 Produkte)

Synonyme: Arc-1 antikoerper, CD324 antikoerper, CDHE antikoerper, ECAD antikoerper, LCAM antikoerper, UVO antikoerper, E-cadherin antikoerper, cdh1 antikoerper, si:dz180o5.2 antikoerper, uvo antikoerper, lcam antikoerper, E-Cad antikoerper, XBcad antikoerper, l-cam antikoerper, xcdh1 antikoerper, cdhc-A antikoerper, xb-cad antikoerper, XTCAD-1 antikoerper, c-cadherin antikoerper, cadherin-1 antikoerper, uvomorulin antikoerper, XB-cadherin antikoerper, arc-1 antikoerper, cd324 antikoerper, cdhe antikoerper, ecad antikoerper, AA960649 antikoerper, E-cad antikoerper, Ecad antikoerper, L-CAM antikoerper, Um antikoerper, cadherin 1 antikoerper, si:busm1-180o5.2 antikoerper, cadherin 1, type 1, E-cadherin (epithelial) antikoerper, cadherin 1, type 1 S homeolog antikoerper, CDH1 antikoerper, si:busm1-180o5.2 antikoerper, cdh1 antikoerper, Cdh1 antikoerper, cdh1.S antikoerper

Hintergrund: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin alpha E beta 7 . The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation