E2F6 Antikörper (Middle Region)

Produktnummer ABIN3044189

Produktdetails anti-E2F6 Antikörper

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Bindungsspezifität: AA 162-177, Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: KDCAQQLFEL TDDKEN

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: E2F transcription factor 6
Protein Name: Transcription factor E2F6(E2F-6)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F6(162-177aa KDCAQQLFELTDDKEN), different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Andere Bezeichnung: E2F6 (E2F6 Produkte)

Synonyme: E2F-6 antikoerper, AI462434 antikoerper, E2F6b antikoerper, EMA antikoerper, E2F transcription factor 6 antikoerper, E2F6 antikoerper, E2f6 antikoerper

Hintergrund: E2F6(E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92 % identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.

Synonyms: E2F 6 antibody|E2F binding site modulating activity protein antibody|E2F transcription factor 6 antibody|E2F transcription factor 6 isoform 1 antibody|E2F-6 antibody|E2F6 antibody|E2F6_HUMAN antibody|EMA antibody|MGC111545 antibody|Transcription factor E2F6 antibody

UniProt: O75461