Vinculin Antikörper (N-Term)

Produktnummer ABIN3044168

Produktdetails anti-Vinculin Antikörper

Target: Vinculin (VCL)

Bindungsspezifität: AA 173-188, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: KMIDERQQEL THQEHR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vinculin
Protein Name: Vinculin

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Vinculin Antikörper (ABIN3044168)

Cong, Fu, Zhang, Zhao, Zhang: "Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 210-216, (2018) (PubMed).

Wang, Wang, Qu, Yue, Hu, Zhang: "VEGF Enhances the Migration of MSCs in Neural Differentiation by Regulating Focal Adhesion Turnover." in: Journal of cellular physiology, Vol. 230, Issue 11, pp. 2728-42, (2015) (PubMed).

Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." in: BioMed research international, Vol. 2015, pp. 871202, (2015) (PubMed).

Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." in: Stem cell research & therapy, Vol. 6, pp. 22, (2015) (PubMed).

Lyu, Wang, Wang, Ding, Liu, Yuan, Shi, Wang, Wang, Chen: "Maintaining the pluripotency of mouse embryonic stem cells on gold nanoparticle layers with nanoscale but not microscale surface roughness." in: Nanoscale, Vol. 6, Issue 12, pp. 6959-69, (2014) (PubMed).

Lyu, Hu, Xu, Zhang: "Dynamics of focal adhesions and reorganization of F-actin in VEGF-stimulated NSCs under varying differentiation states." in: Journal of cellular biochemistry, Vol. 114, Issue 8, pp. 1744-59, (2013) (PubMed).

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL (VCL Produkte)

Synonyme: CMD1W antikoerper, CMH15 antikoerper, MV antikoerper, MVCL antikoerper, VCL antikoerper, im:6902790 antikoerper, hm:zehn2160 antikoerper, DDBDRAFT_0186762 antikoerper, DDBDRAFT_0232320 antikoerper, DDB_0186762 antikoerper, DDB_0232320 antikoerper, 9430097D22 antikoerper, AA571387 antikoerper, AI462105 antikoerper, AW545629 antikoerper, CG3299 antikoerper, CT11081 antikoerper, Dmel\\CG3299 antikoerper, Dmvincp antikoerper, EG:103B4.1 antikoerper, Vin antikoerper, Vin2EF antikoerper, vinc antikoerper, LOC100228062 antikoerper, vcl-A antikoerper, vinculin antikoerper, vinculin a antikoerper, vinculin A antikoerper, Vinculin antikoerper, vinculin S homeolog antikoerper, VCL antikoerper, vcla antikoerper, vinA antikoerper, Vcl antikoerper, Vinc antikoerper, CpipJ_CPIJ013873 antikoerper, Bm1_37935 antikoerper, vcl antikoerper, vcl.S antikoerper

Hintergrund: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Synonyms: CMD1W antibody|CMH15 antibody|Metavinculin antibody|MVCL antibody|OTTHUMP00000019861 antibody|OTTHUMP00000019862 antibody|VCL antibody|VINC antibody|VINC_HUMAN antibody|Vinculin antibody

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2