RNF25 Antikörper (C-Term)

Produktnummer ABIN303143

Produktdetails anti-RNF25 Antikörper

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino Acids near the Carboxyl terminus of human RNF25 protein.

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunoprecipitation. Western Blot (1/200-1/2000). ELISA (1/10000-1/40000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Details zu RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Andere Bezeichnung: RNF25 (RNF25 Produkte)

Synonyme: AO7 antikoerper, 0610009H16Rik antikoerper, ring finger protein 25 antikoerper, RNF25 antikoerper, Rnf25 antikoerper

Hintergrund: RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25

Gen-ID: 64320

NCBI Accession: NP_071898

UniProt: Q96BH1