FZD2 Antikörper (Internal Region)

Produktnummer ABIN302136

Produktdetails anti-FZD2 Antikörper

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FZD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Verwendungszweck: Frizzled 2

Sequenz: RYATLEHPFH CPR

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-FZD2 Antikörper (ABIN302136)

Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

Details zu FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Andere Bezeichnung: FZD2 (FZD2 Produkte)

Synonyme: BG01711 antikoerper, CG9739 antikoerper, D-Fz2 antikoerper, D-fz2 antikoerper, DFZ2 antikoerper, DFz-2 antikoerper, DFz2 antikoerper, Dfrz2 antikoerper, Dfz2 antikoerper, Dfz[[2]] antikoerper, Dm Fz2 antikoerper, Dmel\\CG9739 antikoerper, FZ2 antikoerper, Fz2 antikoerper, dFz2 antikoerper, dfz(2) antikoerper, dfz2 antikoerper, dfz2r antikoerper, frz2 antikoerper, fz-2 antikoerper, fz8 antikoerper, zfz2 antikoerper, zg08 antikoerper, cb383 antikoerper, wu:fb70g09 antikoerper, FZD2 antikoerper, fzE2 antikoerper, hFz2 antikoerper, Fz-10 antikoerper, Fzd10 antikoerper, cFz-2 antikoerper, frizzled2 antikoerper, fz2 antikoerper, xfz2 antikoerper, AL033370 antikoerper, AW456835 antikoerper, Fz10 antikoerper, Mfz10 antikoerper, Mfz10a antikoerper, frizzled 2 antikoerper, frizzled class receptor 2 antikoerper, frizzled class receptor 2 S homeolog antikoerper, fz2 antikoerper, fzd2 antikoerper, FZD2 antikoerper, Fzd2 antikoerper, fzd2.S antikoerper

Hintergrund: FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

Gen-ID: 2535, 57265, 64512

NCBI Accession: NP_001457

Pathways: WNT Signalweg