WNT2B Antikörper
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- Target Alle WNT2B Antikörper anzeigen
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser WNT2B Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Spezifität
- Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
- Aufreinigung
- Affinity purified
- Immunogen
- E. coli-derived rmWnt-2b.
- Isotyp
- IgG
- Top Product
- Discover our top product WNT2B Primärantikörper
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- Applikationshinweise
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well. - Kommentare
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Target Species of Antibody: Mouse
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- deionized water
- Konzentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
- Andere Bezeichnung
- WNT2B (WNT2B Produkte)
- Synonyme
- WNT13 antikoerper, XWNT2 antikoerper, wnt2b antikoerper, XWnt-2 antikoerper, Xwnt-2b antikoerper, wnt-2b antikoerper, xwnt2b antikoerper, WNT2B antikoerper, Wnt13 antikoerper, Wnt family member 2B antikoerper, wingless-type MMTV integration site family, member 2Ba antikoerper, Wnt family member 2B L homeolog antikoerper, wingless-type MMTV integration site family, member 2B antikoerper, wingless-type MMTV integration site family, member 2Bb antikoerper, WNT2B antikoerper, Wnt2b antikoerper, wnt2ba antikoerper, wnt2b.L antikoerper, wnt2bb antikoerper
- Hintergrund
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Name/Gene ID: WNT2B
Family: WNT
Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of - Gen-ID
- 7482
- UniProt
- Q93097
- Pathways
- WNT Signalweg
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