LRP6 Antikörper (AA 1547-1560)

Produktnummer ABIN291653

Der Ziege Polyklonal anti-LRP6 Antikörper wird verwendet zum Nachweis von LRP6 in Proben von Human, Ratte, Hund, Schwein, Kaninchen, Hamster, Pferd und Affe. Er wurde validiert für IHC, ELISA und IHC (p).

Kurzübersicht für LRP6 Antikörper (AA 1547-1560) (ABIN291653)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Reaktivität: Human, Ratte, Hund, Schwein, Kaninchen, Hamster, Pferd, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser LRP6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LRP6 Antikörper

Bindungsspezifität: AA 1547-1560

Spezifität: Human LRP6. This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse, Rabbit, Pig (100%) Mouse, Elephant, Bovine, Bat (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CMTSVATAKGYTSDL from an internal region of human LRP6 (NP_002327.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse, Rabbit, Pig (100%), Mouse, Elephant, Bovine, Bat (93%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu LRP6

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Andere Bezeichnung: LRP6

Hintergrund: Name/Gene ID: LRP6

Synonyms: LRP6, ADCAD2, LRP-6

Gen-ID: 4040

NCBI Accession: NP_002327

UniProt: O75581

Pathways: WNT Signalweg, Tube Formation