p53 Antikörper (Alexa Fluor 647)

Produktnummer ABIN2689872

Der Maus Monoklonal Anti-p53-Antikörper wurde für ICS validiert. Er ist geeignet, p53 in Proben von Human zu detektieren. Es sind 8+ Publikationen verfügbar.

Kurzübersicht für p53 Antikörper (Alexa Fluor 647) (ABIN2689872)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Klon: L82

Produktdetails anti-p53 Antikörper (Alexa Fluor 647)

Marke: BD Phosflow™

Produktmerkmale: The p53 protein is critical to regulation of normal cell growth and proliferation and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. Post-translational acetylation of p53 enhances its DNA-binding activity. There are multiple factors that affect p53 acetylation, thereby modulating cellular proliferation and apoptosis. The L82-51 monoclonal antibody recognizes acetylated lysine 382 (acK382) in the C-terminal region of p53. Analysis of p53 (acK382) in lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either treated with 0.4 μM Trichostatin A (Sigma, Cat. No. T8552) plus 0.4 μM Adriamycin (Doxorubicin hydrochloride, Sigma, Cat. No. D1515) for 24 hours (shaded histogram) or untreated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-p53 (acK382). Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control (left blot) and Trichostatin A-plus-Adriamycin-treated (right blot) PBMC. p53 (acK382) is identified as a band of 53 kDa in the treated cells.

BD Phosflow™ Alexa Fluor® 647 Mouse anti-p53 (acK382) - Alexa Fluor® 647 - Clone L82-51 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p53 acetylated Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-p53 Antikörper (Alexa Fluor 647) (ABIN2689872)

Nishino, Wang, Mochizuki-Kashio, Osawa, Nakauchi, Iwama: "Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase." in: PLoS ONE, Vol. 6, Issue 9, pp. e24298, (2011) (PubMed).

Soliman, Riabowol: "After a decade of study-ING, a PHD for a versatile family of proteins." in: Trends in biochemical sciences, Vol. 32, Issue 11, pp. 509-19, (2007) (PubMed).

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, (2005) (PubMed).

Pedeux, Sengupta, Shen, Demidov, Saito, Onogi, Kumamoto, Wincovitch, Garfield, McMenamin, Nagashima, Grossman, Appella, Harris: "ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation." in: Molecular and cellular biology, Vol. 25, Issue 15, pp. 6639-48, (2005) (PubMed).

Hofmann, Möller, Sirma, Zentgraf, Taya, Dröge, Will, Schmitz: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." in: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, (2002) (PubMed).

Saito, Goodarzi, Higashimoto, Noda, Lees-Miller, Appella, Anderson: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, (2002) (PubMed).

Ito, Lai, Zhao, Saito, Hamilton, Appella, Yao: "p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2." in: The EMBO journal, Vol. 20, Issue 6, pp. 1331-40, (2001) (PubMed).

Vaziri, Dessain, Ng Eaton, Imai, Frye, Pandita, Guarente, Weinberg: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." in: Cell, Vol. 107, Issue 2, pp. 149-59, (2001) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund: Synonyms: TP53 (acK382)

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt