EPH Receptor B2 Antikörper

Produktnummer ABIN2689499

Produktdetails anti-EPH Receptor B2 Antikörper

Target: EPH Receptor B2 (EPHB2)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPH Receptor B2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3. Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in regulating cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis. Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 Molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2. Flow cytometric analysis of EphB2 expression on human Colo-205 cells. Cells from the human COLO 205 (Colorectal adenocarcinoma, ATCC CCL-222) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878, dashed line histogram) or Purified Mouse Anti-Human EphB2 antibody (Cat. No. 564698, solid line histogram). The cells were washed and counterstained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms showing EphB2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Immunohistochemical analysis of human EphB2 expression in human placenta. Acetone-fixed frozen human placenta sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat No.550878, Left Image) or Purified Mouse Anti-Human EphB2 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:20x. 564698 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Human EphB2 - Purified - Clone 2H9 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EphB2 Recombinant Protein

Klon: 2H9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-EPH Receptor B2 Antikörper (ABIN2689499)

Jung, Sato, Merlos-Suárez, Barriga, Iglesias, Rossell, Auer, Gallardo, Blasco, Sancho, Clevers, Batlle: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

Foster, Gordon, Cardenas, Veiga-Fernandes, Makinen, Grigorieva, Wilkinson, Blackburn, Richie, Manley, Adams, Kioussis, Coles: "EphB-ephrin-B2 interactions are required for thymus migration during organogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13414-9, (2010) (PubMed).

Mao, Luis, Ross, Silva, Tan, Crowley, Chui, Franz, Senter, Koeppen, Polakis: "EphB2 as a therapeutic antibody drug target for the treatment of colorectal cancer." in: Cancer research, Vol. 64, Issue 3, pp. 781-8, (2004) (PubMed).

Details zu EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EphB2 (EPHB2 Produkte)

Synonyme: CAPB antikoerper, DRT antikoerper, EK5 antikoerper, EPHT3 antikoerper, ERK antikoerper, Hek5 antikoerper, PCBC antikoerper, Tyro5 antikoerper, ERK2 antikoerper, ERT1 antikoerper, MAPK2 antikoerper, P42MAPK antikoerper, PRKM1 antikoerper, PRKM2 antikoerper, p38 antikoerper, p40 antikoerper, p41 antikoerper, p41mapk antikoerper, RGD1564232 antikoerper, Cek5 antikoerper, Drt antikoerper, ETECK antikoerper, Erk antikoerper, Nuk antikoerper, Prkm5 antikoerper, Qek5 antikoerper, Sek3 antikoerper, cek5 antikoerper, ephb2 antikoerper, xEphB2 antikoerper, si:ch73-160e9.1 antikoerper, tpx2 antikoerper, wu:fb09e10 antikoerper, wu:fc64a03 antikoerper, wu:fi18g12 antikoerper, zgc:112296 antikoerper, EPH receptor B2 antikoerper, mitogen-activated protein kinase 1 antikoerper, Eph receptor B2 antikoerper, EPH receptor B2 L homeolog antikoerper, eph receptor B2a antikoerper, eph receptor B2b antikoerper, EPHB2 antikoerper, MAPK1 antikoerper, Ephb2 antikoerper, ephb2.L antikoerper, ephb2a antikoerper, ephb2b antikoerper

Hintergrund: Synonyms: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine