Tumor Protein P53 (TP53) (pSer392) Antikörper

Antigen: Tumor Protein P53 (TP53)

Synonyme: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Epitope: pSer392

Klonalität: Monoklonal (FP3-2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN238404

Produktbeschreibung

Produktmerkmale: Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Weitere Bezeichnung: P53 (TP53)

Swiss-Prot: P04637

Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids378-393 representing the C-terminus of Human p53.

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: FP3-2

Beschreibung: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals andstimulated to activate transcription of specific genes, resulting in expression of p21waf1and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such asBcl-2. The structure of p53 comprises N-terminal transactivation domain, centralDNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. Thereare various phosphorylation sites on p53, of which the phosphorylation at Ser15 isimportant for p53 activation and stabilization.

Spezifität: This p53 antibody FP3. 2 [FPS392] reacts with Human p53 tumour suppressor proteinphosphorylated at CKII site (Ser392). Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rabbit (85%), Canine (85%), Guinea pig (85%),

Anwendungen

Applikationshinweise: Western Blotting: 1 μg/ml. Immunohistochemistry on Paraffin Sections): 10 μg/mlStaining technique: Standard ABC technique (DAB+)Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4°CPositive Tissue: breast carcinoma with high level of wild-type p53. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: PBS, pH~7.4 containing 15 mM Sodium Azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Transkriptionsfaktoren, Phospho-Antikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar