HSP90 Antikörper (Lys294)

Produktnummer ABIN233817

Produktdetails anti-HSP90 Antikörper

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Bindungsspezifität: Lys294

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP90 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  This antibody reacts strongly with the acetylated form of the immunizing peptide and shows minimal reactivity with the non-acetylated form when tested by ELISA.  To date, western blotting shows equivalent staining of lysates either treated or untreated with Trichostatin A (an HDAC inhibitor).  Therefore, western blotting results are not definitive for demonstrating the specificity of this reagent.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-HSP90 Antikörper (ABIN233817)

Manca, Frisbie, LaGrange, Casey, Riethoven, Petrosyan: "The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer." in: Molecular cancer research : MCR, Vol. 17, Issue 1, pp. 225-237, (2019) (PubMed).

Deskin, Lasky, Zhuang, Shan: "Requirement of HDAC6 for activation of Notch1 by TGF-β1." in: Scientific reports, Vol. 6, pp. 31086, (2018) (PubMed).

Mo, Zhao, Liu, Hu, Chen, Pham, Wei, Liu, Zhou, Burgess, Pfaff, Caskey, Wu, Bai, Yang: "Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy." in: Nature communications, Vol. 9, Issue 1, pp. 1007, (2018) (PubMed).

Dedes, Dedes, Imesch, von Bueren, Fink, Fedier: "Acquired vorinostat resistance shows partial cross-resistance to 'second-generation' HDAC inhibitors and correlates with loss of histone acetylation and apoptosis but not with altered HDAC and HAT activities." in: Anti-cancer drugs, Vol. 20, Issue 5, pp. 321-33, (2009) (PubMed).

Details zu HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Andere Bezeichnung: HSP 90 (HSP90 Produkte)

Synonyme: EL52 antikoerper, HSP86 antikoerper, HSP89A antikoerper, HSP90A antikoerper, HSP90N antikoerper, HSPC1 antikoerper, HSPCA antikoerper, HSPCAL1 antikoerper, HSPCAL4 antikoerper, HSPN antikoerper, Hsp89 antikoerper, Hsp90 antikoerper, LAP2 antikoerper, git10 antikoerper, swo1 antikoerper, HSP90 antikoerper, htpG antikoerper, SCBAC25F8.08 antikoerper, 23.m06066 antikoerper, 17.m07646 antikoerper, HSP90-1 antikoerper, 143198_at antikoerper, 83 antikoerper, 83K HSP antikoerper, DMHSP82 antikoerper, E(sev)3A antikoerper, E(sina)2 antikoerper, HSP82 antikoerper, HSP83 antikoerper, ORF1 antikoerper, Su(Raf)3A antikoerper, anon-EST:Liang-2.53 antikoerper, anon-WO0068693 antikoerper, anon-WO0140519.209 antikoerper, clone 2.53 antikoerper, en(lz)3C/4C antikoerper, hsp84 antikoerper, l(3)j5C2 antikoerper, ms(3)08445 antikoerper, stc antikoerper, DmelCG1242 antikoerper, CG1242 antikoerper, Hsp86 antikoerper, Hspca antikoerper, 86kDa antikoerper, 89kDa antikoerper, AL024080 antikoerper, AL024147 antikoerper, Hsp86-1 antikoerper, hsp4 antikoerper, hsp86 antikoerper, hsp89 antikoerper, hsp90 antikoerper, hsp90a antikoerper, hspc1 antikoerper, hspca antikoerper, hspn antikoerper, lap2 antikoerper, Hsp90alpha antikoerper, heat shock protein 90 alpha family class A member 1 antikoerper, heat shock protein Hsp90 antikoerper, Hsp90 chaperone antikoerper, Heat shock protein 90 antikoerper, heat shock protein 90 antikoerper, chaperone protein HtpG antikoerper, molecular chaperone HtpG antikoerper, Heat Shock Protein 90 antikoerper, uncharacterized LOC100384473 antikoerper, Heat shock protein 83 antikoerper, heat shock protein 90, alpha (cytosolic), class A member 1 antikoerper, heat shock protein 90 alpha family class B member 1 antikoerper, heat shock protein 90kDa alpha family class A member 1 L homeolog antikoerper, heat shock protein HSP 90-alpha antikoerper, HSP90AA1 antikoerper, hsp90 antikoerper, HSP90 antikoerper, daf-21 antikoerper, htpG antikoerper, SCO7516 antikoerper, MAP_RS10510 antikoerper, TP04_0646 antikoerper, TP01_0934 antikoerper, APH_RS03525 antikoerper, GbCGDNIH1_0315 antikoerper, MAV_2118 antikoerper, HSP90C antikoerper, BBOV_IV008400 antikoerper, BBOV_III007380 antikoerper, ACICU_00312 antikoerper, ECL_01244 antikoerper, YE105_C1172 antikoerper, pco153543(105) antikoerper, Hsp83 antikoerper, Hsp90aa1 antikoerper, HSP90AB1 antikoerper, hsp90aa1.1.L antikoerper, LOC108698781 antikoerper

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family, the members of which are highly conserved between isoforms and species. Hsp90 functions as a molecular chaperone and has ATPase activity.  Hsp90 is a cytoplasmic protein that forms a homodimer in vivo and interacts with TOM34, AHSA1, HDAC6 and SMYD3.  Several signal transduction pathways depend on Hsp90 function, including pathways involving erbB2, hypoxia sensitivity (Hif1 alpha), and steroid hormone receptors (for example, androgen, progesterone, glucocorticoid, and aryl-hydrocarbon).  Recent reports show that Hsp90 from tumor cells has increased sensitivity to small molecule inhibitors (for example, 17AAG).  The mechanism of the differential sensitivity of Hsp90 from normal versus tumor cells is unknown, although mutation has been ruled out.  One possible mechanism may be differences in post-translational modification of tumor Hsp90.  K294 was found to be acetylated in purified Hsp90 from SkBr3 cells, a breast cancer cell line.
Synonyms: Heat shock protein HSP 90-alpha Gene names Name: HSP90AA1 Synonyms: HSP90A, HSPCA

Gen-ID: 3320, 123678

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals