E-cadherin Antikörper (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 375-631)

Details for Product anti-CDH1 Antibody No. ABIN1858334, Anbieter: Anmelden zum Anzeigen
Antigen
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • cdh1
  • si:dz180o5.2
  • dZ71B9.1
  • si:dz180o5.1
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • CDH1
  • CDH16
  • HPT-1
  • HPT1
  • arc-1
  • cd324
  • cdhe
  • ecad
  • cad17
  • hpt1
  • cdh16
  • hpt-1
  • LI-cadherin
  • cadherin-17
  • b-cadherin
  • cdh1-a
  • cdhp
  • hjmd
  • pcad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • Arc1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • si:busm1-180o5.2
  • cadherin 1, epithelial
  • cadherin 1
  • si:busm1-180o5.1
  • cadherin 17, LI cadherin (liver-intestine)
  • Cadherin-17
  • cadherin 3, type 1, P-cadherin (placental)
  • E-cadherin-like
  • Aerobic running capacity QTL 1
  • CDH1
  • si:busm1-180o5.2
  • cdh1
  • Cdh1
  • si:busm1-180o5.1
  • CDH17
  • cad17
  • cdh17
  • cdh3-a
  • LOC100356548
  • Arunc1
Alternativen
anti-Wild boar (Sus scrofa) E-cadherin Antikörper für Immunocytochemistry
Epitop
AA 375-631
81
33
27
20
19
16
16
15
15
14
13
10
6
6
6
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Wild boar (Sus scrofa), Schwein
790
238
146
40
26
15
9
8
8
6
5
4
3
1
1
1
1
1
1
1
1
Wirt
Kaninchen
532
266
37
22
2
Klonalität
Polyklonal
Konjugat
Dieser E-cadherin Antikörper ist unkonjugiert
42
38
33
23
20
20
16
15
15
15
14
12
12
12
12
11
11
11
9
8
7
7
6
4
3
3
3
3
3
3
2
2
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
623
351
270
200
173
172
158
89
86
38
14
14
13
10
10
7
6
6
4
2
2
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

'Independent Validation' Siegel
Antigen E-cadherin
Chargennummer A20160427539
Validierte Anwendung Immunofluorescence
Positivkontrolle porcine unerupted tooth buds, dental epithelium
Negativkontrolle porcine unerupted tooth buds, mesenchym
Bewertung Passed. The E-caherin antibody ABIN1858334 specifically labels epithelial tissues in porcine unerupted tooth buds in immunofluorescence, consistent with the expression pattern of E-cadherin.
Primärantikörper ABIN1858334
Sekundärantikörper goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025)
Protokoll
  • Harvest unerupted tooth buds from 5 month old pig jaws and wash 3x in phosphate bufferend saline (PBS) (Boston Bioproducts, BM-220).
  • Fix tooth buds in 10% Formalin (Fisher Scientific, 23-245-685) for 24h.
  • Wash 3x in PBS.
  • Place tooth buds through graded ethanol seris for 6h at each concentration: 50%, 70%, 80%, 90%, 100%, 100%, 100%.
  • Place tooth buds in 3x 2h in 100% xylene.
  • Place in molten paraffin 2x (TissuePrep 2 Paraffin, Fisher, T555-2) 12h.
  • Embed tooth buds in paraffin and cool on a 4°C cold plate for 1h.
  • Let set ON at RT.
  • Cut blocks into 6µm sections using a microtome (ThermoFisher Scientific, HM 355S).
  • Use a tissue floating bath to mount sections onto microscope slides.
  • Heat sections on a hot plate for 1h at 45°C for 1h then ON at 55°C.
  • Deparaffinize slide 2x 5 min in 100% xylene.
  • Rehydrate sections in graded ethanol series for 2min for each concentration: 100%, 95%, 70%, 50%.
  • Place slide in H2O for 5min.
  • Transfer slide to a plastic holder filled with warmed 10mM Citric Acid Solution (made from sodium citrate monobasic; Sigma, 71497) pH6.0 and incubate in a steamer for 20min.
  • Cool submerged slide on benchtop for 30min.
  • Wash slide 3x 5min in PBS.
  • Create a hydrophobic barrier on slide around sections with PAP-pen (Vector Labs, H-4000, lot 5138).
  • Place slide in a humidified chamber and block with 5% BSA for 20min at 37°C.
  • Gently remove BSA by tapping slide.
  • Incubate sections with primary antibody
    • rabbit anti-pig E-cadherin antibody (antibodies-online, ABIN1858334, lot A20160408626) diluted to 1:40 in 1% BSA or
    • and mouse anti-pig Vimentin (Santa Cruz Biotechnology, SC-6260, lot G2814) diluted to 1:500 in 1% BSA for 1h at RT.
  • A no primary antibody negative control was incubated in parallel in 1% BSA.
  • Wash in slide 3x 5min in PBS.
  • Incubate sections in secondary antibodies
    • goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025) diluted to 1:100 in 1% BSA and
    • goat anti-mouse AF488 conjugated antibody (ThermoFisher Scientific, A11001, lot 1752514 ) diluted to 1:100 in 1% BSA for 1h at RT.
  • Wash in slide 3x 5min in PBS.
  • Add approximately 10μL of Hard Set Mounting Medium with DAPI (Vector Labs, H-1500, lot ZC1221) for each section and mount cover slip.
Validierungsbilder
Immunofluorescence validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibody (ABIN1858334) A. ABIN1858334 stains the dental epithelium (DE, red) in pig tooth buds whereas the d...
Immunogen CDHE (Phe375-Thr631)
Isotyp IgG
Spezifität The antibody is a rabbit polyclonal antibody raised against CDHE. It has been selected for its ability to recognize CDHE in immunohistochemical staining and western blotting.
Reinigung Antigen-specific affinity chromatography
Andere Bezeichnung Cadherin, Epithelial (CDHE) (CDH1 Antibody Abstract)
Hintergrund Alternative Names: CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Applikationshinweise
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Kommentare

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-E-cadherin Antikörper (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 375-631) (ABIN1858334) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibody